Evidence map›Paper›PMID 25034019›Full record

ArticlePlant physiology2014

The ASH1-RELATED3 SET-domain protein controls cell division competence of the meristem and the quiescent center of the Arabidopsis primary root.

Robert Kumpf, Tage Thorstensen, Mohummad Aminur Rahman, Jefri Heyman, H Zeynep Nenseth, Tim Lammens, Ullrich Herrmann, Ranjan Swarup, Silje Veie Veiseth, Gitika Emberland and 3 more

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In one paragraph

Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 46 citations in OpenAlex.

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  4. Identification of thePlants (Basel, Switzerland) · 2024
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  11. The function of histone lysine methylation related SET domain group proteins in plants.Protein science : a publication of the Protein Society · 2020
    Review
  12. Article
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  14. Review
  15. Article
  16. Focus on roots.Plant physiology · 2014
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 3 countries.

Robert KumpfDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Tage ThorstensenDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Mohummad Aminur RahmanDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Jefri HeymanDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
H Zeynep NensethDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Tim LammensDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Ullrich HerrmannDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Ranjan SwarupDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Silje Veie VeisethDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Gitika EmberlandDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Malcolm J BennettDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Lieven De VeylderDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.).
Reidunn B AalenDepartment of Biosciences, University of Oslo, N-0316 Oslo, Norway (R.K., T.T., M.A.R., H.Z.N., U.H., S.V.V., G.E., R.B.A.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie and Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (J.H., T.L., L.D.V.); andCentre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom (R.S., M.J.B.) reidunn.aalen@imbv.uio.no.
University of Oslo · NOVlaams Instituut voor Biotechnologie · BEUniversity of Nottingham · GB

Funding

Biotechnology and Biological Sciences Research Council BB/G023972/1Biotechnology and Biological Sciences Research Council BB/H020314/1
6 · The paper itself

Abstract

The stem cell niche of the Arabidopsis (Arabidopsis thaliana) primary root apical meristem is composed of the quiescent (or organizing) center surrounded by stem (initial) cells for the different tissues. Initial cells generate a population of transit-amplifying cells that undergo a limited number of cell divisions before elongating and differentiating. It is unclear whether these divisions occur stochastically or in an orderly manner. Using the thymidine analog 5-ethynyl-2'-deoxyuridine to monitor DNA replication of cells of Arabidopsis root meristems, we identified a pattern of two, four, and eight neighboring cells with synchronized replication along the cortical, epidermal, and endodermal cell files, suggested to be daughters, granddaughters, and great-granddaughters of the direct progeny of each stem cell. Markers of mitosis and cytokinesis were not present in the region closest to the transition zone where the cells start to elongate, suggesting that great-granddaughter cells switch synchronously from the mitotic cell cycle to endoreduplication. Mutations in the stem cell niche-expressed ASH1-RELATED3 (ASHR3) gene, encoding a SET-domain protein conferring histone H3 lysine-36 methylation, disrupted this pattern of coordinated DNA replication and cell division and increased the cell division rate in the quiescent center. E2Fa/E2Fb transcription factors controlling the G1-to-S-phase transition regulate ASHR3 expression and bind to the ASHR3 promoter, substantiating a role for ASHR3 in cell division control. The reduced length of the root apical meristem and primary root of the mutant ashr3-1 indicate that synchronization of replication and cell divisions is required for normal root growth and development.

Indexed as

ArabidopsisArabidopsis ProteinsCell DivisionDNA, PlantDNA ReplicationMeristemMutationPlant RootsS PhaseArabidopsis ProteinsDNA, Plant

Identifiers

PMID25034019
PMCPMC4213094
OpenAlexW2119049692

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.