ArticlePharmaceutical research2015
Nanomagnetic activation as a way to control the efficacy of nucleic acid delivery.
Article in Pharmaceutical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Fabrication and characterization of magnetic trimetallic mesoporous nanocomposite: as an effective nanocatalyst for synthesis of bisphenol derivatives.Scientific reports · 2026Article
- Optimization of mouse kidney digestion protocols for single-cell applications.Physiological genomics · 2024Article
- Design, fabrication and characterization of mesoporous yolk-shell nanocomposites as a sustainable heterogeneous nanocatalyst for synthesis of ortho-aminocarbonitrile tetrahydronaphthalenes.Scientific reports · 2023Article
- Magnetite Nanoparticles: Synthesis and Applications in Optics and Nanophotonics.Materials (Basel, Switzerland) · 2022Review
- Experimental Single-Platform Approach to Enhance the Functionalization of Magnetically Targetable Cells.ACS applied bio materials · 2020Article
- Polyethylenimine based magnetic nanoparticles mediated non-viral CRISPR/Cas9 system for genome editing.Scientific reports · 2020Article
- Improved Delivery of CRISPR/Cas9 System Using Magnetic Nanoparticles into Porcine Fibroblast.Molecular biotechnology · 2019Article
- Nano-mediated delivery of double-stranded RNA for gene therapy of glioblastoma multiforme.PloS one · 2019Article
- A Critical Review on Selected External Physical Cues and Modulation of Cell Behavior: Magnetic Nanoparticles, Non-thermal Plasma and Lasers.Journal of functional biomaterials · 2018Review
- Comparative Analysis of Non-viral Transfection Methods in Mouse Embryonic Fibroblast Cells.Journal of biomolecular techniques : JBT · 2017Article
- Generation of Transgenic Porcine Fibroblast Cell Lines Using Nanomagnetic Gene Delivery Vectors.Molecular biotechnology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo explore the potential of magnetofection in delivering pDNA to primary mouse embryonic fibroblasts (PMEFs) and porcine fetal fibroblasts (PFFs) and investigate an effect of magnetic cell labeling on transfection efficacy.
methodsThe formulation and a dose of the magnetic vector were optimized. The efficacy of the procedure was quantified by vector internalization, transgene expression and cell iron loading upon specific labeling with Ab-conjugated magnetic beads or non-specific labeling with MNPs.
resultsUp to sixty percent of PMEF and PFF cells were transfected at low pDNA doses of 4-16 pg pDNA/cell. Specific labeling of the PMEFs with MNPs, resulted in a 3- and 2-fold increase in pDNA internalization upon magnetofection and lipofection, respectively, that yielded a 2-4-fold increase in percent of transgene-expressing cells. Non-specific cell labeling had no effect on the efficacy of the reporter expression, despite the acquisition of similar magnetic moments per cell. In PFFs, specific magnetic labeling of the cell surface receptors inhibited internalization and transfection efficacy.
conclusionsMagnetic labeling of cell-surface receptors combined with the application of an inhomogenous magnetic field (nanomagnetic activation) can affect the receptor-mediated internalization of delivery vectors and be used to control nucleic acid delivery to cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.