Evidence map›Paper›PMID 25033406›Full record

ArticlePLoS pathogens2014

Real-time imaging reveals the dynamics of leukocyte behaviour during experimental cerebral malaria pathogenesis.

Saparna Pai, Jim Qin, Lois Cavanagh, Andrew Mitchell, Fatima El-Assaad, Rohit Jain, Valery Combes, Nicholas H Hunt, Georges E R Grau, Wolfgang Weninger

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
10.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 71 citations in OpenAlex.

  1. Antigen-experienced airway CD8Science advances · 2026
    Article
  2. Article
  3. Immunophenotyping of Leukocytes in Brain in Hypothyroid Mice.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  4. Review
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Perivascular macrophages create an intravascular niche for CD8Clinical & translational immunology · 2021
    Article
  11. Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021
    Review
  12. Article
  13. Mediterranean Diet: Lipids, Inflammation, and Malaria Infection.International journal of molecular sciences · 2020
    Review
  14. Article
  15. Article
  16. Imaging the dynamic recruitment of monocytes to the blood-brain barrier and specific brain regions duringProceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  17. Monocyte-derived dendritic cells in malaria.Current opinion in microbiology · 2019
    Review
  18. Review
  19. Article
  20. The Liver-StageFrontiers in immunology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Saparna PaiImmune Imaging Laboratory, The Centenary Institute, Newtown, Sydney, New South Wales, Australia; Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
Jim QinImmune Imaging Laboratory, The Centenary Institute, Newtown, Sydney, New South Wales, Australia.
Lois CavanaghImmune Imaging Laboratory, The Centenary Institute, Newtown, Sydney, New South Wales, Australia; Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
Andrew MitchellImmune Imaging Laboratory, The Centenary Institute, Newtown, Sydney, New South Wales, Australia.
Fatima El-AssaadVascular Immunology Unit, Discipline of Pathology, Sydney Medical School, University of Sydney, Camperdown, Sydney, New South Wales, Australia.
Rohit JainImmune Imaging Laboratory, The Centenary Institute, Newtown, Sydney, New South Wales, Australia; Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
Valery CombesSydney Medical School, University of Sydney, Sydney, New South Wales, Australia; Vascular Immunology Unit, Discipline of Pathology, Sydney Medical School, University of Sydney, Camperdown, Sydney, New South Wales, Australia.
Nicholas H HuntSydney Medical School, University of Sydney, Sydney, New South Wales, Australia; Molecular Immunopathology Unit, Discipline of Pathology, Sydney Medical School and Bosch Institute, University of Sydney, Camperdown, Sydney, New South Wales, Australia.
Georges E R GrauSydney Medical School, University of Sydney, Sydney, New South Wales, Australia; Vascular Immunology Unit, Discipline of Pathology, Sydney Medical School, University of Sydney, Camperdown, Sydney, New South Wales, Australia.
Wolfgang WeningerImmune Imaging Laboratory, The Centenary Institute, Newtown, Sydney, New South Wales, Australia; Discipline of Dermatology, University of Sydney, Sydney, New South Wales, Australia; Department of Dermatology, Royal Prince Alfred Hospital, Camperdown, Sydney, New South Wales, Australia.
University of Sydney · AUCentenary Institute · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During experimental cerebral malaria (ECM) mice develop a lethal neuropathological syndrome associated with microcirculatory dysfunction and intravascular leukocyte sequestration. The precise spatio-temporal context in which the intravascular immune response unfolds is incompletely understood. We developed a 2-photon intravital microscopy (2P-IVM)-based brain-imaging model to monitor the real-time behaviour of leukocytes directly within the brain vasculature during ECM. Ly6C(hi) monocytes, but not neutrophils, started to accumulate in the blood vessels of Plasmodium berghei ANKA (PbA)-infected MacGreen mice, in which myeloid cells express GFP, one to two days prior to the onset of the neurological signs (NS). A decrease in the rolling speed of monocytes, a measure of endothelial cell activation, was associated with progressive worsening of clinical symptoms. Adoptive transfer experiments with defined immune cell subsets in recombinase activating gene (RAG)-1-deficient mice showed that these changes were mediated by Plasmodium-specific CD8(+) T lymphocytes. A critical number of CD8(+) T effectors was required to induce disease and monocyte adherence to the vasculature. Depletion of monocytes at the onset of disease symptoms resulted in decreased lymphocyte accumulation, suggesting reciprocal effects of monocytes and T cells on their recruitment within the brain. Together, our studies define the real-time kinetics of leukocyte behaviour in the central nervous system during ECM, and reveal a significant role for Plasmodium-specific CD8(+) T lymphocytes in regulating vascular pathology in this disease.

Indexed as

CD8-Positive T-LymphocytesEndothelial CellsMalaria, CerebralMonocytesAnimalsMiceMice, KnockoutMicroscopy, FluorescencePlasmodium berghei

Identifiers

PMID25033406
PMCPMC4102563
OpenAlexW2044568142

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.