ArticleNature protocols2014
Quantitative and unbiased analysis of directional persistence in cell migration.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 240 papers.
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Who cites it
240 citing papers in PubMed.
- The Nance-Horan syndrome protein NHS regulates cell migration persistence by organizing WAVE and N-WASP nucleation-promoting factor complexes.The Journal of biological chemistry · 2026Article
- EDNRB-dependent endothelin signaling reduces proliferation and promotes proneural-to-mesenchymal transition in gliomas.Molecular oncology · 2026Article
- PDGF-Stimulated Corneal Keratocyte Motility and Migration Patterns on Aligned Collagen Fibrils Are Inhibited by Decorin.International journal of molecular sciences · 2026Article
- Rapid chromosome movements persist within nuclear protrusions in Arabidopsis thaliana meiocytes.Protoplasma · 2026Article
- IqgD is a Rac1-interacting IQGAP required for efficient growth of Dictyostelium discoideum on bacterial lawns.Cell communication and signaling : CCS · 2026Article
- CD44 restricts EGFR mobility to polarize cytoskeletal signalling modules driving bleb-based migration.Nature cell biology · 2026Article
- Mechanical cues regulateMaterials today. Bio · 2026Article
- Tilorone suppresses TGF-β-driven fibroblast activation and restores BMP-Smad1/5/8 signaling: implications for laryngotracheal fibrosis.Journal of translational medicine · 2026Article
- Single-Cell-Resolution Fate Mapping Reveals Embryonic Venous Origins of Fenestrated Hindbrain Choroid Plexus Vasculature.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Context-dependent effect of glucocorticoid receptor activity shapes ovarian cancer cell plasticity and therapy response.Molecular cancer · 2026Article
- Cold atmospheric plasma promotes migration persistence, through induced HBiophysical journal · 2026Article
- Identification of PKN2 and MOB4 as Coordinators of Collective Cell Migration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.Antioxidants (Basel, Switzerland) · 2026Article
- TGFβ induces an atypical EMT to evade immune mechanosurveillance in lung adenocarcinoma dormant metastasis.Nature cancer · 2026Article
- Adherent cells sustain membrane tension gradients independently of migration.Nature communications · 2025Article
- Inhibition of YB-1 phosphorylation enhances cisplatin activity and disrupts cell division in pleural mesothelioma.British journal of cancer · 2025Article
- Cellular elasticity drives mechano-adaptation against fluid shear stress.Journal of cell science · 2025Article
- A Rho GTPase-effector ensemble governs cell migration behavior.Nature communications · 2025Article
- Dynamic rigidity changes enable rapid cell migration on soft substrates.Nature communications · 2025Article
- Article
180 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism by which cells control directional persistence during migration is a major question. However, the common index measuring directional persistence, namely the ratio of displacement to trajectory length, is biased, particularly by cell speed. An unbiased method is to calculate direction autocorrelation as a function of time. This function depends only on the angles of the vectors tangent to the trajectory. This method has not been widely used, because it is more difficult to compute. Here we discuss biases of the classical index and introduce a custom-made open-source computer program, DiPer, which calculates direction autocorrelation. In addition, DiPer also plots and calculates other essential parameters to analyze cell migration in two dimensions: it displays cell trajectories individually and collectively, and it calculates average speed and mean square displacements (MSDs) to assess the area explored by cells over time. This user-friendly program is executable through Microsoft Excel, and it generates plots of publication-level quality. The protocol takes ∼15 min to complete. We have recently used DiPer to analyze cell migration of three different mammalian cell types in 2D cultures: the mammary carcinoma cell line MDA-MB-231, the motile amoeba Dictyostelium discoideum and fish-scale keratocytes. DiPer can potentially be used not only for random migration in 2D but also for directed migration and for migration in 3D (direction autocorrelation only). Moreover, it can be used for any types of tracked particles: cellular organelles, bacteria and whole organisms.
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