ArticleBioconjugate chemistry2014
Homogeneous bispecifics by disulfide bridging.
Article in Bioconjugate chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Self-Reporting Therapeutic Protein Nanoparticles.ACS applied materials & interfaces · 2024Article
- On-Demand Thio-Succinimide Hydrolysis for the Assembly of Stable Protein-Protein Conjugates.Journal of the American Chemical Society · 2024Article
- Chemical generation of checkpoint inhibitory T cell engagers for the treatment of cancer.Nature chemistry · 2023Article
- Modular Chemical Construction of IgG-like Mono- and Bispecific Synthetic Antibodies (SynAbs).ACS central science · 2023Article
- Enhancing Dual-State Emission in Maleimide Fluorophores through Fluorocarbon Functionalisation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Dual reactivity disulfide bridging reagents; enabling new approaches to antibody fragment bioconjugation.Chemical science · 2022Article
- Chemical and Enzymatic Methods for Post-Translational Protein-Protein Conjugation.Journal of the American Chemical Society · 2022Review
- π-Clamp-Mediated Homo- and Heterodimerization of Single-Domain Antibodies via Site-Specific Homobifunctional Conjugation.Journal of the American Chemical Society · 2022Article
- Enabling the next steps in cancer immunotherapy: from antibody-based bispecifics to multispecifics, with an evolving role for bioconjugation chemistry.RSC chemical biology · 2022Review
- Pyrocinchonimides Conjugate to Amine Groups on Proteins via Imide Transfer.Bioconjugate chemistry · 2020Article
- Highly homogeneous antibody modification through optimisation of the synthesis and conjugation of functionalised dibromopyridazinediones.Organic & biomolecular chemistry · 2018Article
- A detergent-based procedure for the preparation of IgG-like bispecific antibodies in high yield.Scientific reports · 2016Article
- Site-Selective Disulfide Modification of Proteins: Expanding Diversity beyond the Proteome.Chemistry (Weinheim an der Bergstrasse, Germany) · 2016Review
- Fluorescent BAPAD Dendrimeric Antigens Are Efficiently Internalized by Human Dendritic Cells.Polymers · 2016Article
- Preclinical evaluation of a novel CEA-targeting near-infrared fluorescent tracer delineating colorectal and pancreatic tumors.International journal of cancer · 2015Article
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Authors and funding
6 authors.
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Abstract
We report on a chemical platform to generate site-specific, homogeneous, antibody-antibody conjugates by targeting and bridging disulfide bonds. A bispecific antibody construct was produced in good yield through simple reduction and bridging of antibody fragment disulfide bonds, using a readily synthesized bis-dibromomaleimide cross-linker. Binding activity of antibodies was maintained, and in vitro binding of target antigens was observed. This technology is demonstrated through linking scFv and Fab antibody fragments, showing its potential for the construction of a diverse range of bispecifics.
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Registered trials
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