ArticleBMJ (Clinical research ed.)1989
Genetic analysis of DNA from single human oocytes: a model for preimplantation diagnosis of cystic fibrosis.
Article in BMJ (Clinical research ed.), 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 101 citations in OpenAlex.
- Concurrent Preimplantation Genetic Testing and Competence Assessment of Human Embryos by Transcriptome Sequencing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Whole Genome Amplification in Preimplantation Genetic Testing in the Era of Massively Parallel Sequencing.International journal of molecular sciences · 2022Review
- The politics of human embryo research and the motivation to achieve PGD.Reproductive biomedicine online · 2011Article
- Knowledge, attitudes, and clinical experience of physicians regarding preimplantation genetic diagnosis for hereditary cancer predisposition syndromes.Familial cancer · 2010Article
- The use of hydrolysis and hairpin probes in real-time PCR.Molecular biotechnology · 2003Review
- Multiplex genotype determination at a large number of gene loci.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Review: preimplantation diagnosis of inherited disease.Journal of inherited metabolic disease · 1996Review
- Optimal polymerase chain reaction amplification for preimplantation diagnosis in cystic fibrosis (delta F508)BMJ (Clinical research ed.) · 1995Article
- Reliability of polymerase chain reaction (PCR) analysis of single cells for preimplantation genetic diagnosis.Journal of assisted reproduction and genetics · 1994Article
- Preimplantation diagnosis.The Western journal of medicine · 1993Review
- A new source of polymorphic DNA markers for sperm typing: analysis of microsatellite repeats in single cells.American journal of human genetics · 1992Article
- Sequencing heavy- and light-chain variable genes of single B-hybridoma cells by total enzymatic amplification.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.Nucleic acids research · 1992Article
- Reliability of gender determination using the polymerase chain reaction (PCR) for single cells.Journal of in vitro fertilization and embryo transfer : IVF · 1991Article
- Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Research on the human conceptus and its regulation in Britain today.Journal of the Royal Society of Medicine · 1990Review
- The preimplantation genetic diagnosis of genetic diseases.Journal of in vitro fertilization and embryo transfer : IVF · 1990Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Gene sequences in human oocytes were studied to investigate the possibility of diagnosing inherited or sporadic genetic disease before implantation after in vitro fertilisation. By specific amplification the possibility of analysing the DNA from single human oocytes for a specific gene was shown, and genotypes for markers closely linked to cystic fibrosis and Duchenne muscular dystrophy were determined. Single oocytes were used to approximate the total amount of DNA present in a single cell taken for biopsy from a 4-16 cell blastocyst. With a new technique for specific DNA amplification, the polymerase chain reaction, these data can be obtained within several hours of cell isolation. Extreme care must be taken to avoid any contamination of the sample with DNA from other sources. With this technique genotyping for single gene disorders is feasible with an accuracy and on a time scale that would allow implantation of the zygote after in vitro fertilisation without freezing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.