Evidence map›Paper›PMID 25031598›Full record

ArticleIranian endodontic journal2014

Effect of Mineral Trioxide Aggregate, Calcium-Enriched Mixture Cement and Mineral Trioxide Aggregate with Disodium Hydrogen Phosphate on BMP-2 Production.

Negin Ghasemi, Saeed Rahimi, Mehrdad Lotfi, Jafar Solaimanirad, Shahriar Shahi, Hajar Shafaie, Amin Salem Milani, Sahar Shakuie, Vahid Zand, Majid Abdolrahimi

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In one paragraph

Article in Iranian endodontic journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 1 pooled it
6.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.

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  17. Iranian endodontic journal · 2017
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  19. Effect of different mixing methods on the physical properties of Portland cement.Journal of clinical and experimental dentistry · 2016
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Negin GhasemiDepartment of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
Saeed RahimiDental and Periodontal Research Center, Department of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
Mehrdad LotfiResearch Center for Pharmaceutical Nanotechnology and Department of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
Jafar SolaimaniradDepartment of Anatomy and Histology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Shahriar ShahiDental and Periodontal Research Center, Department of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
Hajar ShafaieDepartment of Anatomy and Histology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Amin Salem MilaniDental and Periodontal Research Center, Department of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
Sahar ShakuieDepartment of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
Vahid ZandDepartment of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
Majid AbdolrahimiDental and Periodontal Research Center, Department of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
Tabriz University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionOne of the hypotheses regarding the calcification induction by mineral trioxide aggregate (MTA) is the involvement of transforming growth factor-Beta (TGF-β) super family. Calcium-enriched mixture (CEM) cement is one of the endodontic biomaterials with clinical applications similar to MTA. The aim of the present in vitro study was to compare the induction of bone morphogenic protein-2 (BMP-2) by a combination of disodium hydrogen phosphate (DSHP) and tooth colored ProRoot MTA (WMTA), to that of CEM cement and WMTA. METHODS AND MATERIALS: Human gingival fibroblasts (HGFs) were obtained from the attached gingiva of human premolars. HGFs were cultured in Dulbecco's Modified Eagle's medium, supplemented with 10% fetal calf serum, penicillin, and streptomycin. Cells in groups 1, 2 and 3 were exposed to WMTA, CEM and WMTA+DSHP discs, respectively. The fourth group served as the control. After 72 h of exposure, HGF viability was determined by Mosmann's tetrazolium toxicity (MTT) assay. BMP-2 levels in cell-free culture media were determined by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using the one-way ANOVA, followed by the post hoc Games-Howell test for BMP-2 and post hoc Tukey's test for the results of MTT assay.

resultsCellular viability was significantly higher in group 3 compared to the other groups (P<0.05); however, CEM and WMTA did not exhibit significant differences (P=0.08). The control group exhibited significantly higher cellular viability in comparison to the other groups of the study (P<0.05). The highest and lowest protein production rates were observed in the WMTA (3167±274.46 pg/mL) and WMTA+DSHP (1796±839.49 pg/mL) groups, respectively. There were no significant differences between the control, WMTA and CEM groups (P>0.05).

conclusionWMTA and CEM did not exhibit any significant differences in terms of inducing BMP-2 production; however, incorporation of DSHP into WMTA resulted in a decrease in the induction of this protein.

Indexed as

Bone Morphogenic Protein-2Calcium-Enriched Mixture CementDisodium Hydrogen PhosphateFibroblastsMineral Trioxide AggregateTransforming Growth Factor-Beta

Identifiers

PMID25031598
PMCPMC4099956
OpenAlexW1688875320

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.