Evidence map›Paper›PMID 25031577›Full record

ArticleIranian journal of reproductive medicine2014

Molecular and serologic diagnostic approaches; the prevalence of herpes simplex in idiopathic men infertile.

Nasser Amirjannati, Farhad Yaghmaei, Mohammad Mehdi Akhondi, Mahboubeh Nasiri, Hamed Heidari-Vala, Zahra Sehhat

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Iranian journal of reproductive medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 9 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Nasser AmirjannatiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Farhad YaghmaeiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Mohammad Mehdi AkhondiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Mahboubeh NasiriMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Hamed Heidari-ValaNanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Zahra SehhatReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Academic Center for Education, Culture and Research · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman pathogens that can cause infertility may also affect sperm count and quality. Viral infections can be considered as direct and/or indirect cause of male factor infertility.

objectiveOur goal was to investigate the prevalence of herpes simplex virus in the semen of infertile men attending the Avicenna Infertility Clinic, and to compare it with the herpes virus serology results. MATERIALS AND

methodsThis cross sectional study was conducted during 2009-2010. Infertile men participating without any clinical signs of infection with herpes simplex virus, and no obvious cause for their infertility were included. Semen and blood samples were used for Polymerase Chain Reaction (PCR) and serologic testing for these people. Two samples were collected: one ml semen sample to verify the existence of genital herpes simplex virus in infertile men, and blood samples of 217 individuals tested for antibodies to herpes simplex virus. Data were analyzed by SPSS 16.

resultsAccording to the PCR results of semen samples the prevalence of herpes simplex in semen was 12% and serologic test showed 3.2% prevalence within blood. Nine to 10% of IgM negative were PCR positive and only 2-3% of IgM positive were PCR positive. Between herpes serologic studies with positive controls and negative controls by using both tests, there was a significant positive relationship (r=0.718 and p<0.001). The relationship between semen PCR test results and serological survey of herpes patients with a negative control in both Pearson and Spearman tests was positive and significant (r=0.229 and p=0.001). Correlation between the PCR results of semen samples with two positive control subjects and a positive IgM test was statistically confirmed (r=0.235 and p<0.001).

conclusionWe recommend that if there is suspicion to herpes simplex as a microorganism that theoretically could impact semen parameters and cause infertility it is prudent to use PCR technique on semen sample rather than ELISA on serum.

Indexed as

Herpes virusIgMInfertilityPolymerase Chain ReactionSemenSerologicSexually transmitted diseases (STD)

Identifiers

PMID25031577
PMCPMC4094658
OpenAlexW2129885117

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.