ArticleMolecular biology of the cell2014
Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- The MO25 protein Pmo25 functions in contractile ring stability and Sid2 localization during cytokinesis.iScience · 2026Article
- Article
- Roles of the MO25 protein Pmo25 in contractile-ring stability and localization of the NDR kinase Sid2 during cytokinesis.bioRxiv : the preprint server for biology · 2025Article
- New mutations in the core Schizosaccharomyces pombe spindle pole body scaffold Ppc89 reveal separable functions in regulating cell division.G3 (Bethesda, Md.) · 2025Article
- The core spindle pole body scaffold Ppc89 links the pericentrin orthologue Pcp1 to the fission yeast spindle pole body via an evolutionarily conserved interface.Molecular biology of the cell · 2024Article
- Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis.Molecular biology of the cell · 2022Article
- Pcp1/pericentrin controls the SPB number in fission yeast meiosis and ploidy homeostasis.The Journal of cell biology · 2022Article
- Roles of Mso1 and the SM protein Sec1 in efficient vesicle fusion during fission yeast cytokinesis.Molecular biology of the cell · 2020Article
- Mechanisms of chromosome biorientation and bipolar spindle assembly analyzed by computational modeling.eLife · 2020Article
- Spatio-temporal regulation of nuclear division by Aurora B kinase Ipl1 in Cryptococcus neoformans.PLoS genetics · 2019Article
- Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.Molecular biology of the cell · 2018Article
- Centrosome Remodelling in Evolution.Cells · 2018Review
- Review
- Molecular model of fission yeast centrosome assembly determined by superresolution imaging.The Journal of cell biology · 2017Article
- Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast.Science advances · 2017Article
- Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis.Molecular biology of the cell · 2016Article
- Duplication of the Yeast Spindle Pole Body Once per Cell Cycle.Molecular and cellular biology · 2016Review
- Roles of the TRAPP-II Complex and the Exocyst in Membrane Deposition during Fission Yeast Cytokinesis.PLoS biology · 2016Article
- Sbg1 Is a Novel Regulator for the Localization of the β-Glucan Synthase Bgs1 in Fission Yeast.PloS one · 2016Article
- Real-Time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells.Methods in molecular biology (Clifton, N.J.) · 2016Article
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
Centrosomes play critical roles in the cell division cycle and ciliogenesis. Sfi1 is a centrin-binding protein conserved from yeast to humans. Budding yeast Sfi1 is essential for the initiation of spindle pole body (SPB; yeast centrosome) duplication. However, the recruitment and partitioning of Sfi1 to centrosomal structures have never been fully investigated in any organism, and the presumed importance of the conserved tryptophans in the internal repeats of Sfi1 remains untested. Here we report that in fission yeast, instead of doubling abruptly at the initiation of SPB duplication and remaining at a constant level thereafter, Sfi1 is gradually recruited to SPBs throughout the cell cycle. Like an sfi1Δ mutant, a Trp-to-Arg mutant (sfi1-M46) forms monopolar spindles and exhibits mitosis and cytokinesis defects. Sfi1-M46 protein associates preferentially with one of the two daughter SPBs during mitosis, resulting in a failure of new SPB assembly in the SPB receiving insufficient Sfi1. Although all five conserved tryptophans tested are involved in Sfi1 partitioning, the importance of the individual repeats in Sfi1 differs. In summary, our results reveal a link between the conserved tryptophans and Sfi1 partitioning and suggest a revision of the model for SPB assembly.
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