ArticleMolecular biology of the cell2014
SERCaMP: a carboxy-terminal protein modification that enables monitoring of ER calcium homeostasis.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Molecular mechanisms of programmed cell death in methamphetamine-induced neuronal damage.Frontiers in pharmacology · 2022Pooled it
- Nanoplastics Impair Neuroimmune Integrity Via Cellular Retention and Multiple Organelle Stress.Journal of hazardous materials advances · 2026Article
- Human immunodeficiency virus 1 glycoprotein 120 induces endoplasmic reticulum stress in neurons.Cell death & disease · 2025Article
- Excitotoxic glutamate levels cause the secretion of resident endoplasmic reticulum proteins.Journal of neurochemistry · 2024Article
- A transgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis.Transgenic research · 2023Article
- Prosaposin PS18 reduces dopaminergic neurodegeneration in a 6-hydroxydopamine rat model of Parkinson's disease.Scientific reports · 2023Article
- Palmitate and thapsigargin have contrasting effects on ER membrane lipid composition and ER proteostasis in neuronal cells.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2022Article
- Identification of ER/SR resident proteins as biomarkers for ER/SR calcium depletion in skeletal muscle cells.Orphanet journal of rare diseases · 2022Article
- Caffeine and MDMA (Ecstasy) Exacerbate ER Stress Triggered by Hyperthermia.International journal of molecular sciences · 2022Article
- Pathways Linking Nicotinamide Adenine Dinucleotide Phosphate Production to Endoplasmic Reticulum Protein Oxidation and Stress.Frontiers in molecular biosciences · 2022Review
- CaFaculty reviews · 2022Review
- The Pancreatic ß-cell Response to Secretory Demands and Adaption to Stress.Endocrinology · 2021Review
- Neurotoxicity of methamphetamine: Main effects and mechanisms.Experimental neurology · 2021Review
- Altered neuronal physiology, development, and function associated with a common chromosome 15 duplication involving CHRNA7.BMC biology · 2021Article
- The Function of KDEL Receptors as UPR Genes in Disease.International journal of molecular sciences · 2021Review
- A target-agnostic screen identifies approved drugs to stabilize the endoplasmic reticulum-resident proteome.Cell reports · 2021Article
- Computational Modeling of C-Terminal Tails to Predict the Calcium-Dependent Secretion of Endoplasmic Reticulum Resident Proteins.Frontiers in chemistry · 2021Article
- Trophic activities of endoplasmic reticulum proteins CDNF and MANF.Cell and tissue research · 2020Review
- Preclinical model systems of ryanodine receptor 1-related myopathies and malignant hyperthermia: a comprehensive scoping review of works published 1990-2019.Orphanet journal of rare diseases · 2020Article
- Identification of drug modifiers for RYR1-related myopathy using a multi-species discovery pipeline.eLife · 2020Article
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5 authors.
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Abstract
Endoplasmic reticulum (ER) calcium homeostasis is disrupted in diverse pathologies, including neurodegeneration, cardiovascular diseases, and diabetes. Temporally defining calcium dysregulation during disease progression, however, has been challenging. Here we describe secreted ER calcium-monitoring proteins (SERCaMPs), which allow for longitudinal monitoring of ER calcium homeostasis. We identified a carboxy-terminal modification that is sufficient to confer release of a protein specifically in response to ER calcium depletion. A Gaussia luciferase (GLuc)-based SERCaMP provides a simple and sensitive method to monitor ER calcium homeostasis in vitro or in vivo by analyzing culture medium or blood. GLuc-SERCaMPs revealed ER calcium depletion in rat primary neurons exposed to various ER stressors. In vivo, ER calcium disruption in rat liver was monitored over several days by repeated sampling of blood. Our results suggest that SERCaMPs will have broad applications for the long-term monitoring of ER calcium homeostasis and the development of therapeutic approaches to counteract ER calcium dysregulation.
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