ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
The ion transport peptide is a new functional clock neuropeptide in the fruit fly Drosophila melanogaster.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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61 citing papers in PubMed, 114 citations in OpenAlex.
- A Tale of Two States: Similarities and Differences in Neuropeptide Signaling in Larval and Adult Drosophila.The Journal of comparative neurology · 2026Review
- Cell-type-specific circadian and light-responsive transcriptional dynamics in adultProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Ion transport peptide regulates larval body water balance via a receptor guanylyl cyclase iniScience · 2026Article
- Anticipatory capture of circulating peptidergic vesicles in a clock neuron.Molecular biology of the cell · 2026Article
- Sleep regulation inFrontiers in neuroscience · 2026Review
- Anti-diuretic hormone ITP signals via a guanylate cyclase receptor to modulate systemic homeostasis ineLife · 2025Article
- The Never Given 2022 Pittendrigh/Aschoff Lecture: The Clock Network in the Brain-Insights From Insects.Journal of biological rhythms · 2025Article
- Correlation between circadian and photoperiodic latitudinal clines inOpen biology · 2025Article
- Neuropeptide-Dependent Spike Time Precision and Plasticity in Circadian Output Neurons.The European journal of neuroscience · 2025Article
- A brief history of insect neuropeptide and peptide hormone research.Cell and tissue research · 2025Review
- Transcriptome and Neuroendocrinome Responses to Environmental Stress in the Model and Pest InsectInternational journal of molecular sciences · 2025Article
- The circadian genes are required in DAL neurons forFrontiers in neuroscience · 2025Article
- Temperature cues are integrated in a flexible circadian neuropeptidergic feedback circuit to remodel sleep-wake patterns in flies.PLoS biology · 2024Article
- Light and dopamine impact two circadian neurons to promote morning wakefulness.Current biology : CB · 2024Article
- Light and dopamine impact two circadian neurons to promote morning wakefulness.bioRxiv : the preprint server for biology · 2024Article
- Alcohol sensitivity and tolerance encoding in sleep regulatory circadian neurons in Drosophila.Addiction biology · 2023Article
- The head mesodermal cell couples FMRFamide neuropeptide signaling with rhythmic muscle contraction in C. elegans.Nature communications · 2023Article
- Pigment-dispersing factor is present in circadian clock neurons of pea aphids and may mediate photoperiodic signalling to insulin-producing cells.Open biology · 2023Article
- Alcohol tolerance encoding in sleep regulatory circadian neurons in Drosophila.bioRxiv : the preprint server for biology · 2023Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The clock network of Drosophila melanogaster expresses various neuropeptides, but a function in clock-mediated behavioral control was so far only found for the neuropeptide pigment dispersing factor (PDF). Here, we propose a role in the control of behavioral rhythms for the ion transport peptide (ITP), which is expressed in the fifth small ventral lateral neuron, one dorsal lateral neuron, and in only a few nonclock cells in the brain. Immunocytochemical analyses revealed that ITP, like PDF, is most probably released in a rhythmic manner at projection terminals in the dorsal protocerebrum. This rhythm continues under constant dark conditions, indicating that ITP release is clock controlled. ITP expression is reduced in the hypomorph mutant Clk(AR), suggesting that ITP expression is regulated by CLOCK. Using a genetically encoded RNAi construct, we knocked down ITP in the two clock cells and found that these flies show reduced evening activity and increased nocturnal activity. Overexpression of ITP with two independent timeless-GAL4 lines completely disrupted behavioral rhythms, but only slightly dampened PER cycling in important pacemaker neurons, suggesting a role for ITP in clock output pathways rather than in the communication within the clock network. Simultaneous knockdown (KD) of ITP and PDF made the flies hyperactive and almost completely arrhythmic under constant conditions. Under light-dark conditions, the double-KD combined the behavioral characteristics of the single-KD flies. In addition, it reduced the flies' sleep. We conclude that ITP and PDF are the clock's main output signals that cooperate in controlling the flies' activity rhythms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.