Evidence map›Paper›PMID 25030892›Full record

ArticleReproduction (Cambridge, England)2014

Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues.

Eran Altman, Pamela Yango, Radwa Moustafa, James F Smith, Peter C Klatsky, Nam D Tran

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproduction (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
6.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 39 citations in OpenAlex.

  1. Article
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  5. Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Eran AltmanDepartment of ObstetricsGynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USAHelen Schneider Hospital for WomenRabin Medical Center, Petah Tikva, IsraelDepartment of UrologyUniversity of California, San Francisco, San Francisco, California, USADepartment of Obstetrics and GynecologyAlbert Einstein University, Bronx, New York, USA Department of ObstetricsGynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USAHelen Schneider Hospital for WomenRabin Medical Center, Petah Tikva, IsraelDepartment of UrologyUniversity of California, San Francisco, San Francisco, California, USADepartment of Obstetrics and GynecologyAlbert Einstein University, Bronx, New York, USA.
Pamela YangoDepartment of ObstetricsGynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USAHelen Schneider Hospital for WomenRabin Medical Center, Petah Tikva, IsraelDepartment of UrologyUniversity of California, San Francisco, San Francisco, California, USADepartment of Obstetrics and GynecologyAlbert Einstein University, Bronx, New York, USA.
Radwa MoustafaDepartment of ObstetricsGynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USAHelen Schneider Hospital for WomenRabin Medical Center, Petah Tikva, IsraelDepartment of UrologyUniversity of California, San Francisco, San Francisco, California, USADepartment of Obstetrics and GynecologyAlbert Einstein University, Bronx, New York, USA.
James F SmithDepartment of ObstetricsGynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USAHelen Schneider Hospital for WomenRabin Medical Center, Petah Tikva, IsraelDepartment of UrologyUniversity of California, San Francisco, San Francisco, California, USADepartment of Obstetrics and GynecologyAlbert Einstein University, Bronx, New York, USA.
Peter C KlatskyDepartment of ObstetricsGynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USAHelen Schneider Hospital for WomenRabin Medical Center, Petah Tikva, IsraelDepartment of UrologyUniversity of California, San Francisco, San Francisco, California, USADepartment of Obstetrics and GynecologyAlbert Einstein University, Bronx, New York, USA.
Nam D TranDepartment of ObstetricsGynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USAHelen Schneider Hospital for WomenRabin Medical Center, Petah Tikva, IsraelDepartment of UrologyUniversity of California, San Francisco, San Francisco, California, USADepartment of Obstetrics and GynecologyAlbert Einstein University, Bronx, New York, USA trann@obgyn.ucsf.edu.
University of California San Francisco Medical Center · USUniversity of California, San Francisco · US

Funding

NICHD NIH HHS L50 HD073094
6 · The paper itself

Abstract

Autologous spermatogonial stem cell (SSC) transplantation is a potential therapeutic modality for patients with azoospermia following cancer treatment. For this promise to be realized, definitive membrane markers of prepubertal and adult human SSCs must be characterized in order to permit SSC isolation and subsequent expansion. This study further characterizes the markers of male gonocytes, prespermatogonia, and SSCs in humans. Human fetal, prepubertal, and adult testicular tissues were analyzed by confocal microscopy, fluorescence-activated cell sorting, and qRT-PCR for the expression of unique germ cell membrane markers. During male fetal development, THY1 and KIT (C-Kit) are transient markers of gonocytes but not in prespermatogonia and post-natal SSCs. Although KIT expression is detected in gonocytes, THY1 expression is also detected in the somatic component of the fetal testes in addition to gonocytes. In the third trimester of gestation, THY1 expression shifts exclusively to the somatic cells of the testes where it continues to be detected only in the somatic cells postnatally. In contrast, SSEA4 expression was only detected in the gonocytes, prespermatogonia, SSCs, and Sertoli cells of the fetal and prepubertal testes. After puberty, SSEA4 expression can only be detected in primitive spermatogonia. Thus, although THY1 and KIT are transient markers of gonocytes, SSEA4 is the only common membrane marker of gonocytes, prespermatogonia, and SSCs from fetal through adult human development. This finding is essential for the isolation of prepubertal and adult SSCs, which may someday permit fertility preservation and reversal of azoospermia following cancer treatment.

Indexed as

Adult Stem CellsAge FactorsBiomarkersCell SeparationFlow CytometryHumansMalePhenotypeProto-Oncogene Proteins c-kitSpermatogoniaStage-Specific Embryonic AntigensTestisThy-1 AntigensTime FactorsBiomarkersProto-Oncogene Proteins c-kitstage-specific embryonic antigen-4Stage-Specific Embryonic AntigensThy-1 Antigens

Identifiers

PMID25030892
PMCPMC4599365
OpenAlexW2136638707

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.