ArticleNature2014
The cancer glycocalyx mechanically primes integrin-mediated growth and survival.
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Article in Nature, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 366 papers.
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366citing papers in PubMed
29.6field-weighted citation impact, top 1% of its field
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Who cites it
366 citing papers in PubMed, 691 citations in OpenAlex.
- Single-molecule force spectroscopy of ligand-receptor mechanics at extracellular vesicle biointerfaces.Journal of materials chemistry. B · 2026Article
- Engineering CAR-T cells to remodel the mucin-rich cancer cell glycocalyx.bioRxiv : the preprint server for biology · 2026Article
- Review
- From Glycocode to Precision Oncology: Therapeutic Strategies Targeting Aberrant O-GalNAc Glycosylation in Cancer.Chemical reviews · 2026Review
- The microenvironment dictates glyco-immune surveillance via HSF1-mediated metabolism.Science advances · 2026Article
- Incorporation of Novel Synthetic Glycolipids in Liposomal Nanoparticles Affects Opsonization and In Vivo Clearance.Angewandte Chemie (International ed. in English) · 2026Article
- Organization and Dynamics of Focal Adhesions: Light Diffraction Analysis of Cellular Adhesion on Nanopatterned Surfaces.ACS applied materials & interfaces · 2026Article
- Review
- Mechanical Signaling From ECMs to Nuclear Envelopes in Cell Transitions and Fate Decisions: A Review and Perspective on Adhesion-Niche-Based Anti-EndMT and Anti-EMT.Annals of biomedical engineering · 2026Review
- Force Spectroscopy by Atomic Force Microscopy as Indicator for Cellular Microplastic Uptake.International journal of molecular sciences · 2026Article
- Membrane Protein Glycosylation Revisited: Functional Dynamics and Emerging Clinical Insights.International journal of molecular sciences · 2026Review
- Hyaluronic acid and tissue mechanics orchestrate mammalian digit tip regeneration.Science (New York, N.Y.) · 2026Article
- Acute high shear stress enhances fungal cell/substrate adhesion.Microbiology spectrum · 2026Article
- The protease cathepsin K can debulk the cancer glycocalyx.The Journal of biological chemistry · 2026Article
- Glycocalyx micro- and nanodomains in cell-cell and cell-matrix interactions revealed by enhanced click chemistry.Nature communications · 2026Article
- Regulation of cell dynamics by rapid integrin transport through the biosynthetic pathway.The Journal of cell biology · 2026Article
- Cancer cells metastasize in several types of cancer via two distinct routes, probably based on mechanical signals.Frontiers in cell and developmental biology · 2026Review
- Tumor Metastasis in the Microcirculation.Advances in experimental medicine and biology · 2026Review
- Glycocalyx at the host-virus interface: a double-edged sword in virus infection and tissue damage.Frontiers in molecular biosciences · 2026Review
- Compression-induced NF-κB activation sustains tumor cell survival in confinement by detoxifying aldehydes and promotes metastasis.Nature communications · 2025Article
306 more citing papers are in PubMed but not listed here.
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Authors and funding
20 authors at 7 institutions in 2 countries.
Matthew J Paszek1] Department of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, California 94143, USA [2] Bay Area Physical Sciences-Oncology Program, University of California, Berkeley, California 94720, USA [3] School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14853, USA [4] Laboratory for Atomic and Solid State Physics and Kavli Institute at Cornell for Nanoscale Science, Cornell University, Ithaca, New York 14853, USA.
Christopher C DuFort1] Department of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, California 94143, USA [2] Bay Area Physical Sciences-Oncology Program, University of California, Berkeley, California 94720, USA.
Olivier Rossier1] Interdisciplinary Institute for Neuroscience, University of Bordeaux, UMR 5297, F-33000 Bordeaux, France [2] CNRS, Interdisciplinary Institute for Neuroscience, University of Bordeaux, UMR 5297, F-33000 Bordeaux, France.
Russell Bainer1] Department of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, California 94143, USA [2] Bay Area Physical Sciences-Oncology Program, University of California, Berkeley, California 94720, USA.
Janna K MouwDepartment of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, California 94143, USA.
Kamil Godula1] Department of Chemistry, University of California, Berkeley, California 94720, USA [2] The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA [3] Department of Chemistry and Biochemistry, University of California, San Diego, California 92093, USA.
Jason E HudakDepartment of Chemistry, University of California, Berkeley, California 94720, USA.
Jonathon N LakinsDepartment of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, California 94143, USA.
Amanda C Wijekoon1] Department of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, California 94143, USA [2] Bay Area Physical Sciences-Oncology Program, University of California, Berkeley, California 94720, USA.
Luke Cassereau1] Department of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, California 94143, USA [2] Bay Area Physical Sciences-Oncology Program, University of California, Berkeley, California 94720, USA.
Matthew G Rubashkin1] Department of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, California 94143, USA [2] Bay Area Physical Sciences-Oncology Program, University of California, Berkeley, California 94720, USA.
Mark J Magbanua1] Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, California 94143, USA [2] Division of Hematology/Oncology, University of California, San Francisco, California 94143, USA.
Kurt S ThornDepartment of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA.
Michael W DavidsonNational High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida 32310, USA.
Hope S Rugo1] Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, California 94143, USA [2] Division of Hematology/Oncology, University of California, San Francisco, California 94143, USA.
John W Park1] Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, California 94143, USA [2] Division of Hematology/Oncology, University of California, San Francisco, California 94143, USA.
Daniel A HammerDepartments of Chemical and Biomolecular Engineering and Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Grégory Giannone1] Interdisciplinary Institute for Neuroscience, University of Bordeaux, UMR 5297, F-33000 Bordeaux, France [2] CNRS, Interdisciplinary Institute for Neuroscience, University of Bordeaux, UMR 5297, F-33000 Bordeaux, France.
Carolyn R Bertozzi1] Department of Chemistry, University of California, Berkeley, California 94720, USA [2] Department of Molecular Biology, University of California, Berkeley, California 94720, USA [3] Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA.
Valerie M Weaver1] Department of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, California 94143, USA [2] Bay Area Physical Sciences-Oncology Program, University of California, Berkeley, California 94720, USA [3] Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, California 94143, USA [4] Departments of Anatomy and Bioengineering and Therapeutic Sciences and Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California, San Francisco, California 94143, USA.
University of California, San Francisco · USUniversity of California, Berkeley · USUCSF Helen Diller Family Comprehensive Cancer Center · USUniversité de Bordeaux · FRFlorida State University · USPhysical Sciences (United States) · USUniversity of Pennsylvania · US
Funding
TRANS-NETWORK PROJECTSU54CA143836 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI LIPHARDT, JAN T. · 2009 to 2014
$15.1MUniv of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5MInvestigations of Golgi EnzymesR01GM059907 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI BERTOZZI, CAROLYN · 1999 to 2016
$5.9MThe vascular niche in glioma propagation and resistance to therapyU54CA163155 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BERGERS, GABRIELE, WEAVER, VALERIE MARIE · 2011 to 2015
$4.4MRole of Fibroblasts, Myeloid Cells and Matrix in PDACU01CA151925 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI KALLURI, RAGHU, MOSES, HAROLD L · 2010 to 2014
$3.2MSimulation of Lymphocyte Adhesion using Integrated Adhesive DynamicsR01AI082292 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI HAMMER, DANIEL A · 2009 to 2017
$2.8MEnvironmental Effect on the Mammary Gland across the LifespanU01ES019458 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WERB, ZENA · 2010 to 2014
$2.5MCollagen remodeling and tumor progressionR01CA138818 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WEAVER, VALERIE MARIE · 2010 to 2014
$2.0MNeoProteoglycans as synthetic materials for regenerative medicine and bioimagingR00EB013446 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GODULA, KAMIL · 2013 to 2015
$722kSpinning disk confocal / FRAP microscope for quantitative live cell imagingS10RR026758 · NCRR · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WITTMANN, TORSTEN · 2010 to 2010
$500kNeoProteoglycans as synthetic materials for regenerative medicine and bioimagingK99EB013446 · NIBIB · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI GODULA, KAMIL · 2011 to 2012
$165kHoward Hughes Medical InstituteNCI NIH HHS CA138818-01A1NCI NIH HHS R01 CA138818NCI NIH HHS U01 CA151925NCI NIH HHS U54 CA143836NCI NIH HHS U54CA143836-01NCI NIH HHS U54 CA163155NCI NIH HHS U54CA163155-01NCRR NIH HHS S10 RR026758NIAID NIH HHS AI082292-03A1NIAID NIH HHS R01 AI082292NIBIB NIH HHS K99 EB013446NIBIB NIH HHS K99 EB013446-02NIBIB NIH HHS R00 EB013446NIEHS NIH HHS 1U01 ES019458-01NIEHS NIH HHS U01 ES019458NIGMS NIH HHS 2R01GM059907-13NIGMS NIH HHS GM59907NIGMS NIH HHS R01 GM059907NIGMS NIH HHS T32 GM066698
Abstract
Malignancy is associated with altered expression of glycans and glycoproteins that contribute to the cellular glycocalyx. We constructed a glycoprotein expression signature, which revealed that metastatic tumours upregulate expression of bulky glycoproteins. A computational model predicted that these glycoproteins would influence transmembrane receptor spatial organization and function. We tested this prediction by investigating whether bulky glycoproteins in the glycocalyx promote a tumour phenotype in human cells by increasing integrin adhesion and signalling. Our data revealed that a bulky glycocalyx facilitates integrin clustering by funnelling active integrins into adhesions and altering integrin state by applying tension to matrix-bound integrins, independent of actomyosin contractility. Expression of large tumour-associated glycoproteins in non-transformed mammary cells promoted focal adhesion assembly and facilitated integrin-dependent growth factor signalling to support cell growth and survival. Clinical studies revealed that large glycoproteins are abundantly expressed on circulating tumour cells from patients with advanced disease. Thus, a bulky glycocalyx is a feature of tumour cells that could foster metastasis by mechanically enhancing cell-surface receptor function.
Indexed as
AnimalsBreastCell Line, TumorCell ProliferationCell SurvivalFibroblastsGlycocalyxGlycoproteinsHumansImmobilized ProteinsIntegrinsMiceMolecular Targeted TherapyMucin-1Neoplasm MetastasisNeoplasmsGlycoproteinsImmobilized ProteinsIntegrinsMucin-1Receptors, Cell Surface
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.