ArticleBlood2014
Human CD68 promoter GFP transgenic mice allow analysis of monocyte to macrophage differentiation in vivo.
Article in Blood, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
72 citing papers in PubMed, 108 citations in OpenAlex.
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- Two axolotl-adapted cell-ablation platforms reveal macrophage-dependent processes essential for spinal-cord and skeletal regeneration.bioRxiv : the preprint server for biology · 2026Article
- Microglial PGC-1α alleviates synaptic damage and cognitive impairments following anesthesia and surgery by suppressing excessive synaptic pruning in aged mice.International journal of biological sciences · 2026Article
- Development of a Novel Murine Model of In-Stent Neoatherosclerosis.Journal of the American Heart Association · 2025Article
- Cardiac lymphatics retain LYVE-1-dependent macrophages during neonatal mouse heart regeneration.Nature cardiovascular research · 2025Article
- Redefining the role of IL-18 in post-surgical recovery and sepsis: a key mediator of inflammation resolution.Journal of translational medicine · 2025Article
- Activated Microglia Mediate the Motor Neuron-, Synaptic Denervation- and Muscle Wasting-Changes in Burn Injured Mice.Journal of cachexia, sarcopenia and muscle · 2025Article
- Comprehensive analysis of myeloid reporter mice.bioRxiv : the preprint server for biology · 2025Article
- MicroRNAs regulate alveolar macrophage homeostasis and its function in lung fibrosis.Frontiers in immunology · 2025Article
- Alternations in inflammatory macrophage niche drive phenotypic and functional plasticity of Kupffer cells.Nature communications · 2024Article
- Neither injury induced macrophages within the nerve, nor the environment created by Wallerian degeneration is necessary for enhanced in vivo axon regeneration after peripheral nerve injury.Journal of neuroinflammation · 2024Article
- Ly6CCells · 2024Article
- Monocytes release cystatin F dimer to associate with Aβ and aggravate amyloid pathology and cognitive deficits in Alzheimer's disease.Journal of neuroinflammation · 2024Article
- Identification of a distinct cluster of GDF15Frontiers in immunology · 2024Article
- Human Bone Marrow Mesenchymal Stem Cells Promote the M2 Phenotype in Macrophages Derived from STEMI Patients.International journal of molecular sciences · 2023Article
- Direct neuronal conversion of microglia/macrophages reinstates neurological function after stroke.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Apoptotic cell fragments locally activate tingible body macrophages in the germinal center.Cell · 2023Article
- Peripheral nerve repair is associated with augmented cross-tissue inflammation following vascularized composite allotransplantation.Frontiers in immunology · 2023Article
- Interleukin-10 disrupts liver repair in acetaminophen-induced acute liver failure.Frontiers in immunology · 2023Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
Abstract
The recruitment of monocytes and their differentiation into macrophages at sites of inflammation are key events in determining the outcome of the inflammatory response and initiating the return to tissue homeostasis. To study monocyte trafficking and macrophage differentiation in vivo, we have generated a novel transgenic reporter mouse expressing a green fluorescent protein (GFP) under the control of the human CD68 promoter. CD68-GFP mice express high levels of GFP in both monocyte and embryo-derived tissue resident macrophages in adult animals. The human CD68 promoter drives GFP expression in all CD115(+) monocytes of adult blood, spleen, and bone marrow; we took advantage of this to directly compare the trafficking of bone marrow-derived CD68-GFP monocytes to that of CX3CR1(GFP) monocytes in vivo using a sterile zymosan peritonitis model. Unlike CX3CR1(GFP) monocytes, which downregulate GFP expression on differentiation into macrophages in this model, CD68-GFP monocytes retain high-level GFP expression for 72 hours after differentiation into macrophages, allowing continued cell tracking during resolution of inflammation. In summary, this novel CD68-GFP transgenic reporter mouse line represents a powerful resource for analyzing monocyte mobilization and monocyte trafficking as well as studying the fate of recruited monocytes in models of acute and chronic inflammation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.