Evidence map›Paper›PMID 25028573›Full record

ArticleProteome science2014

Proteomic analysis of Cucumis sativus cotyledons after glucohexaose treatment as a part of ROS accumulation related resistance mechanism.

Yuhan Hao, Chunmei Lin, Haiyan Fan, Yang Yu, Ning Li, Shaoli Chen

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Article in Proteome science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
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2citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yuhan HaoCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, PR China ; State Key Laboratory of Genetic Engineering and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
Chunmei LinCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, PR China.
Haiyan FanCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, PR China ; Key Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University, Shenyang 110866, PR China.
Yang YuCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, PR China.
Ning LiCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, PR China.
Shaoli ChenFruit and Silkworm Administrative Station of Liaoning Province, Shenyang 110866, PR China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGlucohexaose is a safe farm chemical used for pathogen prevention, which can induce systemic acquired resistance in cucumber.

resultsWe found that glucohexaose treatment of cucumber plant induced an accumulation of the reactive oxidative species (ROS). Histochemistry showed sharp increases in O(2-) and H2O2 5 h after glucohexaose treatment. After 5 h, the O(2-) content decreased to a normal level, but the H2O2 content remained at a high level 10 h after glucohexaose treatment. And antioxidant enzymes were also changed after glucohexaose treatment. We also investigated the relationship between ROS accumulation and glucohexaose-induced proteome alteration using 2D electrophoresis coupled with MS/MS. 54 protein spots, which enhanced expression under glucohexaose treatment but suppressed the expression by application of DPI and DMTU, have been identified.

conclusionOur study showed the accumulation of ROS is a part of mechanism of glucohexaose induced resistance in cucumber cotyledons. The up-regulated proteins identified by MS such as PP2C and antioxidation proteins are important in ROS signaling. It will be interesting to find out the regulatory mechanism underlying the induction of these proteins via ROS, and provide some clues to the mechanism of glucohexaose-induced resistance.

Indexed as

Cucumis sativusGlucohexaoseProteomeReactive oxygen species

Identifiers

PMID25028573
PMCPMC4098955

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