ArticleProceedings of the National Academy of Sciences of the United States of America2014
Genomic characterization of three unique Dehalococcoides that respire on persistent polychlorinated biphenyls.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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63 citing papers in PubMed, 204 citations in OpenAlex.
- Rhamnolipid-augmented medium enhances growth ofApplied and environmental microbiology · 2026Article
- Cofactor binding interactions with reductive dehalogenase shape cobamide utilization in organohalide-respiring bacteria.Communications biology · 2026Article
- Mechanism of FThe journal of physical chemistry. B · 2026Article
- The PceA-orthologous reductive dehalogenase of Dehalococcoides mccartyi CBDB1 is involved in 1,2,4-trichlorobenzene respiration.FEMS microbiology ecology · 2026Article
- Dechlorination of mixed chlorinated organophosphate esters (V6 and TCEP) and associated reductive dehalogenase gene expression byApplied and environmental microbiology · 2026Article
- Microbially-mediated halogenation and dehalogenation cycling of organohalides in the ocean.Nature communications · 2025Article
- Draft genomes ofMicrobiology resource announcements · 2025Article
- Evidence of microbial reductive dehalogenation in deep-sea cold seeps and its implications for biogeochemical cycles.Microbiome · 2025Article
- Comparative proteogenomics reveals ecological and evolutionary insights into the organohalide-respiringApplied and environmental microbiology · 2025Article
- Unveiling organohalide respiration potential in River Nile sediments via 16S rRNA gene amplicon sequencing of endogenous bacterial communities.BMC microbiology · 2025Article
- Unravelling biosynthesis and biodegradation potentials of microbial dark matters in hypersaline lakes.Environmental science and ecotechnology · 2024Article
- Metagenomic 16S rRNA analysis and predictive functional profiling revealed intrinsic organohalides respiration and bioremediation potential in mangrove sediment.BMC microbiology · 2024Article
- Global prevalence of organohalide-respiring bacteria dechlorinating polychlorinated biphenyls in sewage sludge.Microbiome · 2024Article
- Effects of humic electron mediators on reductive dechlorination of polychlorinated biphenyl by consortia enriched from terrestrial and marine environments.Frontiers in microbiology · 2024Article
- Dehalococcoides mccartyi strain NIT01 grows more stably in vessels made of pure titanium rather than the stainless alloy SUS304.Environmental microbiology reports · 2023Article
- Enrichment of Aerobic and Anaerobic Hydrocarbon-Degrading Bacteria from Multicontaminated Marine Sediment in Mar Piccolo Site (Taranto, Italy).Microorganisms · 2023Article
- Genome-Resolved Metagenomics and Metatranscriptomics Reveal Insights into the Ecology and Metabolism of Anaerobic Microbial Communities in PCB-Contaminated Sediments.Environmental science & technology · 2023Article
- Generation of zero-valent sulfur from dissimilatory sulfate reduction in sulfate-reducing microorganisms.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Salinity determines performance, functional populations, and microbial ecology in consortia attenuating organohalide pollutants.The ISME journal · 2023Article
- Resuscitation-Promoting Factor Accelerates Enrichment of Highly Active Tetrachloroethene/Polychlorinated Biphenyl-Dechlorinating Cultures.Applied and environmental microbiology · 2023Article
3 more citing papers are in PubMed but not listed here.
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7 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Fastidious anaerobic bacteria play critical roles in environmental bioremediation of halogenated compounds. However, their characterization and application have been largely impeded by difficulties in growing them in pure culture. Thus far, no pure culture has been reported to respire on the notorious polychlorinated biphenyls (PCBs), and functional genes responsible for PCB detoxification remain unknown due to the extremely slow growth of PCB-respiring bacteria. Here we report the successful isolation and characterization of three Dehalococcoides mccartyi strains that respire on commercial PCBs. Using high-throughput metagenomic analysis, combined with traditional culture techniques, tetrachloroethene (PCE) was identified as a feasible alternative to PCBs to isolate PCB-respiring Dehalococcoides from PCB-enriched cultures. With PCE as an alternative electron acceptor, the PCB-respiring Dehalococcoides were boosted to a higher cell density (1.2 × 10(8) to 1.3 × 10(8) cells per mL on PCE vs. 5.9 × 10(6) to 10.4 × 10(6) cells per mL on PCBs) with a shorter culturing time (30 d on PCE vs. 150 d on PCBs). The transcriptomic profiles illustrated that the distinct PCB dechlorination profile of each strain was predominantly mediated by a single, novel reductive dehalogenase (RDase) catalyzing chlorine removal from both PCBs and PCE. The transcription levels of PCB-RDase genes are 5-60 times higher than the genome-wide average. The cultivation of PCB-respiring Dehalococcoides in pure culture and the identification of PCB-RDase genes deepen our understanding of organohalide respiration of PCBs and shed light on in situ PCB bioremediation.
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