ArticleInvestigative ophthalmology & visual science2014
Corneal wound healing, a newly identified function of CAP37, is mediated by protein kinase C delta (PKCδ).
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- The Role of Nutraceuticals in Age-Related Ocular Diseases.Molecules (Basel, Switzerland) · 2025Review
- Review
- Topical Adoptive Transfer of Plasmacytoid Dendritic Cells for Corneal Wound Healing.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A Novel Therapeutic Approach to Corneal Alkaline Burn Model by Targeting Fidgetin-Like 2, a Microtubule Regulator.Translational vision science & technology · 2021Article
- Antimicrobial Peptides Derived from the Immune Defense Protein CAP37 Inhibit TLR4 Activation by S100A9.Investigative ophthalmology & visual science · 2020Article
- The utilization of an ocular wound chamber on corneal epithelial wounds.Clinical ophthalmology (Auckland, N.Z.) · 2018Article
- Bioactive Antimicrobial Peptides as Therapeutics for Corneal Wounds and Infections.Advances in wound care · 2017Review
- Effect of Cationic Antimicrobial Protein CAP37 on Cytokine Profile during Corneal Wound Healing.Journal of ocular diseases and therapeutics · 2017Article
- The antimicrobial protein, CAP37, is upregulated in pyramidal neurons during Alzheimer's disease.Histochemistry and cell biology · 2015Article
- The Novel Functions of the PLC/PKC/PKD Signaling Axis in G Protein-Coupled Receptor-Mediated Chemotaxis of Neutrophils.Journal of immunology research · 2015Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe neutrophil-derived granular protein, CAP37, an innate immune system molecule, has antibiotic and immunomodulatory effects on host cells, including corneal epithelial cells. We previously showed that CAP37 modulates corneal epithelial cell migration, adhesion, and proliferation, and that protein kinase C delta (PKCδ) mediates CAP37-induced chemotaxis of these cells. The objective of this study was to investigate the hypothesis that CAP37 facilitates corneal wound healing through the PKC signaling pathway.
methodsThe standard "scratch" assay performed on monolayers of corneal epithelial cells was used to measure the in vitro effect of CAP37 on wound closure. In vivo wound healing in response to CAP37 was measured using a mouse corneal epithelium abrasion model. In vitro and in vivo wound closure were monitored over 48 hours. The PKCδ was visualized during wound closure in cell monolayers and corneal epithelium by immunohistochemistry. The importance of PKCδ in CAP37-induced corneal wound healing was assessed by siRNA.
resultsWe found that CAP37 accelerated wound closure in vitro and in vivo. Maximal closure occurred with concentrations of CAP37 between 250 and 500 ng/mL. Topical applications on mouse cornea led to re-epithelialization of the cornea by 24 hours. Immunohistochemistry of in vitro and in vivo wounds revealed a local increase of PKCδ along the wound edge in CAP37-treated cell monolayers and corneas, compared to untreated controls. CAP37-induced corneal wound healing was significantly reduced in vivo upon treatment with PKCδ siRNA.
conclusionsThese findings support the hypothesis that CAP37 facilitates corneal wound healing through the activation of PKCδ.
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