ArticleHistochemistry and cell biology2014
The tyrosine hydroxylase 2 (TH2) system in zebrafish brain and stress activation of hypothalamic cells.
Article in Histochemistry and cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- A genetic model for development, physiology and behavior of catecholamine-deficient zebrafish larvae.Communications biology · 2026Article
- Immunohistochemical Profile of a Conspicuously Organized Structure in the Dorsal Forebrain of the Peacock Gudgeon, Tateurndina ocellicauda.The Journal of comparative neurology · 2025Article
- Multimodal sensory overload in dopamine-deficient larval zebrafish leads to paradoxical kinesia.Research square · 2025Article
- Integrative Roles of Dopamine Pathway and Calcium Channels Reveal a Link between Schizophrenia and Opioid Use Disorder.International journal of molecular sciences · 2023Article
- Parkinson's disease model in zebrafish using intraperitoneal MPTP injection.Frontiers in neuroscience · 2023Article
- Deletion of lrrk2 causes early developmental abnormalities and age-dependent increase of monoamine catabolism in the zebrafish brain.PLoS genetics · 2021Article
- Neurotrophins Time Point Intervention after Traumatic Brain Injury: From Zebrafish to Human.International journal of molecular sciences · 2021Review
- Neuromodulation and Behavioral Flexibility in Larval Zebrafish: From Neurotransmitters to Circuits.Frontiers in molecular neuroscience · 2021Article
- Molecular Characterization and Expression of Cytochrome P450 Aromatase in Atlantic Croaker Brain: Regulation by Antioxidant Status and Nitric Oxide Synthase During Hypoxia Stress.Frontiers in physiology · 2021Article
- Hypothalamic Dopamine Neurons Control Sensorimotor Behavior by Modulating Brainstem Premotor Nuclei in Zebrafish.Current biology : CB · 2020Article
- Kisspeptin-1 regulates forebrain dopaminergic neurons in the zebrafish.Scientific reports · 2020Article
- Cerebral Dopamine Neurotrophic Factor Regulates Multiple Neuronal Subtypes and Behavior.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Embryonic exposure to valproic acid affects the histaminergic system and the social behaviour of adult zebrafish (Danio rerio).British journal of pharmacology · 2018Article
- Comparative analysis of monoaminergic cerebrospinal fluid-contacting cells in Osteichthyes (bony vertebrates).The Journal of comparative neurology · 2017Article
- A Novel Developmental Role for Dopaminergic Signaling to Specify Hypothalamic Neurotransmitter Identity.The Journal of biological chemistry · 2016Article
- Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation.Current biology : CB · 2016Article
- The Histochemistry and Cell Biology pandect: the year 2014 in review.Histochemistry and cell biology · 2015Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two tyrosine hydroxylases (TH1 and TH2) are found in teleost fish, but no antibodies are available for TH2 protein to analyze the detailed structure of the system. We generated antibodies targeting TH2 and used them to characterize the TH2-producing cells in larval and adult zebrafish brain. The rabbit antisera reliably detected two bands corresponding to TH1 and TH2 close to 55 kDa in brain homogenates. The antisera detected neurons in brain nuclei which express th1 and th2 mRNA; knockdown of th2 expression by morpholino oligonucleotide injection abolished both the th2 mRNA signal and immunoreactivity with the rabbit antisera in TH2 cells. Double staining of samples with the rabbit antiserum made against TH2 and a monoclonal antibody which detects only TH1 allowed identification of cell groups expressing either one of the proteins. Cell groups in preoptic area, anterior, intermediate, and posterior part of the paraventricular organ contained neurons stained with the new TH2 antisera but not with the characterized monoclonal TH1 antibody. Neurons immunoreactive for TH2 and 5-HT were distinct. In situ hybridization for the mRNA of the immediate early gene c-fos combined with TH1/TH2 immunohistochemistry was used to characterize the cells of the zebrafish brain reacting to handling stress and a noxious chemical stimulus. Strong upregulation of c-fos expression was detected in hypothalamic nuclei containing TH2 cells, but few of the c-fos-expressing cells were positive for TH2, suggesting that these stressors do not directly activate a large proportion of TH2 cells.
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