ArticleBMC microbiology2014
Electrochemical selection and characterization of a high current-generating Shewanella oneidensis mutant with altered cell-surface morphology and biofilm-related gene expression.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 57 citations in OpenAlex.
- Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.Journal of bacteriology · 2026Article
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- A Novel Agro-Waste Formulated Medium Enhanced the Growth of Electrogenic Enterobacter Species Isolated Using Microbial Fuel Cell System: Response Surface Methodology Approach.Environmental microbiology reports · 2025Article
- Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology.Synthetic and systems biotechnology · 2023Article
- Supplementation with Amino Acid Sources Facilitates Fermentative Growth of Shewanella oneidensis MR-1 in Defined Media.Applied and environmental microbiology · 2023Article
- Systematic Full-Cycle Engineering Microbial Biofilms to Boost Electricity Production inResearch (Washington, D.C.) · 2023Article
- Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA fromMicroorganisms · 2022Article
- Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis.Biotechnology for biofuels · 2021Article
- Electroactive microorganisms in bioelectrochemical systems.Nature reviews. Microbiology · 2019Review
- Electricigens in the anode of microbial fuel cells: pure cultures versus mixed communities.Microbial cell factories · 2019Review
- Electrochemically active bacteria sense electrode potentials for regulating catabolic pathways.Nature communications · 2018Article
- Article
- Structures, Compositions, and Activities of Live Shewanella Biofilms Formed on Graphite Electrodes in Electrochemical Flow Cells.Applied and environmental microbiology · 2017Article
- CRP Regulates D-Lactate Oxidation inFrontiers in microbiology · 2017Article
- Transcriptional mechanisms for differential expression of outer membrane cytochrome genes omcA and mtrC in Shewanella oneidensis MR-1.BMC microbiology · 2015Article
- Catabolic and regulatory systems in Shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells.Frontiers in microbiology · 2015Review
- Metabolic Characteristics of a Glucose-Utilizing Shewanella oneidensis Strain Grown under Electrode-Respiring Conditions.PloS one · 2015Article
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundShewanella oneidensis MR-1 exhibits extracellular electron transfer (EET) activity that is influenced by various cellular components, including outer-membrane cytochromes, cell-surface polysaccharides (CPS), and regulatory proteins. Here, a random transposon-insertion mutant library of S. oneidensis MR-1 was screened after extended cultivation in electrochemical cells (ECs) with a working electrode poised at +0.2 V (vs. Ag/AgCl) to isolate mutants that adapted to electrode-respiring conditions and identify as-yet-unknown EET-related factors.
resultsSeveral mutants isolated from the enrichment culture exhibited rough morphology and extraordinarily large colonies on agar plates compared to wild-type MR-1. One of the isolated mutants, designated strain EC-2, produced 90% higher electric current than wild-type MR-1 in ECs and was found to have a transposon inserted in the SO_1860 (uvrY) gene, which encodes a DNA-binding response regulator of the BarA/UvrY two-component regulatory system. However, an in-frame deletion mutant of SO_1860 (∆SO_1860) did not exhibit a similar level of current generation as that of EC-2, suggesting that the enhanced current-generating capability of EC-2 was not simply due to the disruption of SO_1860. In both EC-2 and ∆SO_1860, the transcription of genes related to CPS synthesis was decreased compared to wild-type MR-1, suggesting that CPS negatively affects current generation. In addition, transcriptome analyses revealed that a number of genes, including those involved in biofilm formation, were differentially expressed in EC-2 compared to those in ∆SO_1860.
conclusionsThe present results indicate that the altered expression of the genes related to CPS biosynthesis and biofilm formation is associated with the distinct morphotype and high current-generating capability of strain EC-2, suggesting an important role of these genes in determining the EET activity of S. oneidensis.
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