ArticleStem cells and development2014
Sphere formation permits Oct4 reprogramming of ciliary body epithelial cells into induced pluripotent stem cells.
Article in Stem cells and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Review
- Mechanotransduction Regulates Reprogramming Enhancement in Adherent 3D Keratocyte Cultures.Frontiers in bioengineering and biotechnology · 2021Article
- Nonteratoid Medulloepithelioma Presenting in a 78-Year-Old Male.Ocular oncology and pathology · 2016Article
- Research on induced pluripotent stem cells and the application in ocular tissues.International journal of ophthalmology · 2015Review
- Article
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3 authors.
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Abstract
Somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells by defined sets of transcription factors. We previously described reprogramming of monolayer-cultured adult mouse ciliary body epithelial (CE) cells by Oct4 and Klf4, but not with Oct4 alone. In this study, we report that Oct4 alone is sufficient to reprogram CE cells to iPS cells through sphere formation. Furthermore, we demonstrate that sphere formation induces a partial reprogramming state characterized by expression of retinal progenitor markers, upregulation of reprogramming transcription factors, such as Sall4 and Nanog, demethylation in the promoter regions of pluripotency associated genes, and mesenchymal to epithelial transition. The Oct4-iPS cells maintained normal karyotypes, expressed markers for pluripotent stem cells, and were capable of differentiating into derivatives of all three embryonic germ layers in vivo and in vitro. These findings suggest that sphere formation may render somatic cells more susceptible to reprogramming.
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