ArticleBiochemistry2014
Secondary structure of a conserved domain in an intron of influenza A M1 mRNA.
Article in Biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Locations and structures of influenza A virus packaging-associated signals and other functional elements via an in silico pipeline for predicting constrained features in RNA viruses.PLoS computational biology · 2024Article
- Structural and Functional RNA Motifs of SARS-CoV-2 and Influenza A Virus as a Target of Viral Inhibitors.International journal of molecular sciences · 2023Review
- A Test and Refinement of Folding Free Energy Nearest Neighbor Parameters for RNA Including NJournal of molecular biology · 2022Article
- Nearest neighbor rules for RNA helix folding thermodynamics: improved end effects.Nucleic acids research · 2022Article
- Secondary structure prediction for RNA sequences including NNature communications · 2022Article
- RNA Secondary Structure Motifs of the Influenza A Virus as Targets for siRNA-Mediated RNA Interference.Molecular therapy. Nucleic acids · 2020Article
- In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs.Nucleic acids research · 2019Article
- Precise small-molecule cleavage of an r(CUG) repeat expansion in a myotonic dystrophy mouse model.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Secondary structure of the segment 5 genomic RNA of influenza A virus and its application for designing antisense oligonucleotides.Scientific reports · 2019Article
- Structural and Functional Motifs in Influenza Virus RNAs.Frontiers in microbiology · 2018Review
- A new method for detecting signal regions in ordered sequences of real numbers, and application to viral genomic data.PloS one · 2018Article
- Influenza virus segment 5 (+)RNA - secondary structure and new targets for antiviral strategies.Scientific reports · 2017Article
- A compensatory mutagenesis study of a conserved hairpin in the M gene segment of influenza A virus shows its role in virus replication.RNA biology · 2017Article
- Subtype-specific structural constraints in the evolution of influenza A virus hemagglutinin genes.Scientific reports · 2016Article
- Computational and molecular analysis of conserved influenza A virus RNA secondary structures involved in infectious virion production.RNA biology · 2016Article
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- Bridging the gap between in vitro and in vivo RNA folding.Quarterly reviews of biophysics · 2016Article
- Nuclear Magnetic Resonance-Assisted Prediction of Secondary Structure for RNA: Incorporation of Direction-Dependent Chemical Shift Constraints.Biochemistry · 2015Article
- Structural features of a 3' splice site in influenza a.Biochemistry · 2015Article
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Authors and funding
5 authors.
Funding
Abstract
Influenza A virus utilizes RNA throughout infection. Little is known, however, about the roles of RNA structures. A previous bioinformatics survey predicted multiple regions of influenza A virus that are likely to generate evolutionarily conserved and stable RNA structures. One predicted conserved structure is in the pre-mRNA coding for essential proteins, M1 and M2. This structure starts 79 nucleotides downstream of the M2 mRNA 5' splice site. Here, a combination of biochemical structural mapping, mutagenesis, and NMR confirms the predicted three-way multibranch structure of this RNA. Imino proton NMR spectra reveal no change in secondary structure when 80 mM KCl is supplemented with 4 mM MgCl2. Optical melting curves in 1 M NaCl and in 100 mM KCl with 10 mM MgCl2 are very similar, with melting temperatures ∼14 °C higher than that for 100 mM KCl alone. These results provide a firm basis for designing experiments and potential therapeutics to test for function in cell culture.
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