ArticleAnalytical chemistry2014
Detecting kinase activities from single cell lysate using concentration-enhanced mobility shift assay.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 45 citations in OpenAlex.
- In situ and real-time monitoring of intracellular activities in single live cells using a nanopore probe.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cross-Species Applications of Peptide Substrate Reporters to Quantitative Measurements of Kinase Activity.ACS measurement science au · 2024Article
- Investigation on the Stability of Random Vortices in an Ion Concentration Polarization Layer with Imposed Normal Fluid Flow.Micromachines · 2020Article
- Free Flow Ion Concentration Polarization Focusing (FF-ICPF).Analytical chemistry · 2020Article
- Nanoelectrokinetic bufferchannel-less radial preconcentrator and online extractor by tunable ion depletion layer.Biomicrofluidics · 2019Article
- Ion Concentration Polarization by Bifurcated Current Path.Scientific reports · 2017Article
- Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone.Journal of visualized experiments : JoVE · 2017Article
- Microfluidic Sample Preparation for Single Cell Analysis.Analytical chemistry · 2016Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Electrokinetic preconcentration coupled with mobility shift assays can give rise to very high detection sensitivities. We describe a microfluidic device that utilizes this principle to detect cellular kinase activities by simultaneously concentrating and separating substrate peptides with different phosphorylation states. This platform is capable of reliably measuring kinase activities of single adherent cells cultured in nanoliter volume microwells. We also describe a novel method utilizing spacer peptides that significantly increase separation resolution while maintaining high concentration factors in this device. Thus, multiplexed kinase measurements can be implemented with single cell sensitivity. Multiple kinase activity profiling from single cell lysate could potentially allow us to study heterogeneous activation of signaling pathways that can lead to multiple cell fates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.