ArticlePloS one2014
Dual fluorescent reporter pig for Cre recombination: transgene placement at the ROSA26 locus.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Activating a patient-relevant pathogenic variant of a genetically defined heart disease in a humanized pig model.Lab animal · 2026Article
- A multimodal cross-species comparison of pancreas development.Nature communications · 2025Article
- Advances in large-scale DNA engineering with the CRISPR system.Experimental & molecular medicine · 2025Review
- ZIP13 marks muscle satellite cells and contributes to their quiescent and active phase balance.Scientific reports · 2025Article
- Article
- Endothelial Retargeting of AAV9 In Vivo.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- CRISPR/Cas9-mediated knock-in strategy at the Rosa26 locus in cattle fetal fibroblasts.PloS one · 2022Article
- Germline competent mesoderm: the substrate for vertebrate germline and somatic stem cells?Biology open · 2021Article
- Cas9-expressing chickens and pigs as resources for genome editing in livestock.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Missing Link: Cre Pigs for Cancer Research.Frontiers in oncology · 2021Article
- Highly efficient CRISPR-targeting of the murine Hipp11 intergenic region supports inducible human transgene expression.Molecular biology reports · 2020Article
- Genetically Engineered Pigs to Study Cancer.International journal of molecular sciences · 2020Review
- Endothelial Cell Lineage Analysis Does Not Provide Evidence for EMT in Adult Valve Homeostasis and Disease.Anatomical record (Hoboken, N.J. : 2007) · 2019Article
- Translational Aspects of Adeno-Associated Virus-Mediated Cardiac Gene Therapy.Human gene therapy · 2018Review
- Efficient targeted integration into the bovine Rosa26 locus using TALENs.Scientific reports · 2018Article
- Production of transgenic pigs using aJournal of veterinary science · 2018Article
- Overview of the reporter genes and reporter mouse models.Animal models and experimental medicine · 2018Review
- Genetically engineered pigs as models for human disease.Disease models & mechanisms · 2018Review
- Article
- Discovery of Nigri/nox and Panto/pox site-specific recombinase systems facilitates advanced genome engineering.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We are extending the Cre/loxP site-specific recombination system to pigs, focussing on conditional and tissue-specific expression of oncogenic mutations to model human cancers. Identifying the location, pattern and extent of Cre recombination in vivo is an important aspect of this technology. Here we report pigs with a dual fluorochrome cassette under the control of the strong CAG promoter that switches expression after Cre-recombination, from membrane-targeted tandem dimer Tomato to membrane-targeted green fluorescent protein. The reporter cassette was placed at the porcine ROSA26 locus by conventional gene targeting using primary mesenchymal stem cells, and animals generated by nuclear transfer. Gene targeting efficiency was high, and analysis of foetal organs and primary cells indicated that the reporter is highly expressed and functional. Cre reporter pigs will provide a multipurpose indicator of Cre recombinase activity, an important new tool for the rapidly expanding field of porcine genetic modification.
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