Evidence map›Paper›PMID 25025376›Full record

ArticlePloS one2014

tropiTree: an NGS-based EST-SSR resource for 24 tropical tree species.

Joanne R Russell, Peter E Hedley, Linda Cardle, Siobhan Dancey, Jenny Morris, Allan Booth, David Odee, Lucy Mwaura, William Omondi, Peter Angaine and 6 more

Abstract read
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Forest genetic monitoring: an overview of concepts and definitions.Environmental monitoring and assessment · 2016
    Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Joanne R RussellCell and Molecular Sciences, James Hutton Institute, Invergowrie, Scotland, United Kingdom.
Peter E HedleyCell and Molecular Sciences, James Hutton Institute, Invergowrie, Scotland, United Kingdom.
Linda CardleInformation and Computational Sciences, James Hutton Institute, Invergowrie, Scotland, United Kingdom.
Siobhan DanceyCell and Molecular Sciences, James Hutton Institute, Invergowrie, Scotland, United Kingdom; College of Life Sciences, University of Dundee, Dundee, Scotland, United Kingdom.
Jenny MorrisCell and Molecular Sciences, James Hutton Institute, Invergowrie, Scotland, United Kingdom.
Allan BoothCell and Molecular Sciences, James Hutton Institute, Invergowrie, Scotland, United Kingdom.
David OdeeHeadquarters, Kenya Forestry Research Institute, Nairobi, Kenya; Centre for Ecology & Hydrology at Edinburgh, Centre for Ecology & Hydrology, Penicuik, Scotland, United Kingdom.
Lucy MwauraHeadquarters, World Agroforestry Centre, Nairobi, Kenya.
William OmondiHeadquarters, Kenya Forestry Research Institute, Nairobi, Kenya.
Peter AngaineHeadquarters, Kenya Forestry Research Institute, Nairobi, Kenya.
Joseph MachuaHeadquarters, Kenya Forestry Research Institute, Nairobi, Kenya.
Alice MuchugiHeadquarters, World Agroforestry Centre, Nairobi, Kenya.
Iain MilneInformation and Computational Sciences, James Hutton Institute, Invergowrie, Scotland, United Kingdom.
Roeland KindtHeadquarters, World Agroforestry Centre, Nairobi, Kenya.
Ramni JamnadassHeadquarters, World Agroforestry Centre, Nairobi, Kenya.
Ian K DawsonCell and Molecular Sciences, James Hutton Institute, Invergowrie, Scotland, United Kingdom; Headquarters, World Agroforestry Centre, Nairobi, Kenya.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The development of genetic tools for non-model organisms has been hampered by cost, but advances in next-generation sequencing (NGS) have created new opportunities. In ecological research, this raises the prospect for developing molecular markers to simultaneously study important genetic processes such as gene flow in multiple non-model plant species within complex natural and anthropogenic landscapes. Here, we report the use of bar-coded multiplexed paired-end Illumina NGS for the de novo development of expressed sequence tag-derived simple sequence repeat (EST-SSR) markers at low cost for a range of 24 tree species. Each chosen tree species is important in complex tropical agroforestry systems where little is currently known about many genetic processes. An average of more than 5,000 EST-SSRs was identified for each of the 24 sequenced species, whereas prior to analysis 20 of the species had fewer than 100 nucleotide sequence citations. To make results available to potential users in a suitable format, we have developed an open-access, interactive online database, tropiTree (http://bioinf.hutton.ac.uk/tropiTree), which has a range of visualisation and search facilities, and which is a model for the efficient presentation and application of NGS data.

Indexed as

Genes, PlantBase SequenceCrops, AgriculturalDatabases, GeneticExpressed Sequence TagsHigh-Throughput Nucleotide SequencingMicrosatellite RepeatsSequence Analysis, DNATrees

Identifiers

PMID25025376
PMCPMC4099372

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.