ArticlePloS one2014
Dissecting virus entry: replication-independent analysis of virus binding, internalization, and penetration using minimal complementation of β-galactosidase.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Development of a Live-Cell Imaging Assay to Elucidate Spatiotemporal Dynamics of Extracellular Vesicle Fusion with Target Cells.Journal of extracellular vesicles · 2026Article
- Development of enterovirus transencapsidation assays as tools to understand viral entry.The Journal of general virology · 2025Article
- Imaging flow cytometry reveals the mechanism of equine arteritis virus entry and internalization.Scientific reports · 2025Article
- SARS-CoV-2 Omicron sublineages show comparable cell entry but differential neutralization by therapeutic antibodies.Cell host & microbe · 2022Article
- Cryo-electron tomography of Birbeck granules reveals the molecular mechanism of langerin lattice formation.eLife · 2022Article
- Measuring the subcellular compartmentalization of viral infections by protein complementation assay.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Approaching coronavirus disease 2019: Mechanisms of action of repurposed drugs with potential activity against SARS-CoV-2.Biochemical pharmacology · 2020Review
- Development of HiBiT-Tagged Recombinant Infectious Bronchitis Coronavirus for EfficientFrontiers in microbiology · 2020Article
- Methods for characterizing protein acetylation during viral infection.Methods in enzymology · 2019Article
- Virion Background and Efficiency of Virion Incorporation Determine Susceptibility of Simian Immunodeficiency Virus Env-Driven Viral Entry to Inhibition by IFITM Proteins.Journal of virology · 2017Article
- Interaction of human papillomavirus type 16 particles with heparan sulfate and syndecan-1 molecules in the keratinocyte extracellular matrix plays an active role in infection.The Journal of general virology · 2015Article
- Development of a reporter peptide that catalytically produces a fluorescent signal through α-complementation.Protein science : a publication of the Protein Society · 2015Article
- ATP1A1-mediated Src signaling inhibits coronavirus entry into host cells.Journal of virology · 2015Article
- Coronavirus cell entry occurs through the endo-/lysosomal pathway in a proteolysis-dependent manner.PLoS pathogens · 2014Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies of viral entry into host cells often rely on the detection of post-entry parameters, such as viral replication or the expression of a reporter gene, rather than on measuring entry per se. The lack of assays to easily detect the different steps of entry severely hampers the analysis of this key process in virus infection. Here we describe novel, highly adaptable viral entry assays making use of minimal complementation of the E. coli β-galactosidase in mammalian cells. Enzyme activity is reconstituted when a small intravirion peptide (α-peptide) is complementing the inactive mutant form ΔM15 of β-galactosidase. The method allows to dissect and to independently detect binding, internalization, and fusion of viruses during host cell entry. Here we use it to confirm and extend current knowledge on the entry process of two enveloped viruses: vesicular stomatitis virus (VSV) and murine hepatitis coronavirus (MHV).
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