ArticleJournal of immunology (Baltimore, Md. : 1950)2014
A reporter mouse reveals lineage-specific and heterogeneous expression of IRF8 during lymphoid and myeloid cell differentiation.
Article in Journal of immunology (Baltimore, Md. : 1950), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- A unified multimodal single-cell framework reveals a discrete state model of hematopoiesis in mice.Nature immunology · 2025Article
- Decreased PU.1 expression in mature B cells induces lymphomagenesis.Cancer science · 2024Article
- IRF8 defines the epigenetic landscape in postnatal microglia, thereby directing their transcriptome programs.Nature immunology · 2024Article
- IRF8 configures enhancer landscape in postnatal microglia and directs microglia specific transcriptional programs.bioRxiv : the preprint server for biology · 2024Article
- Circulating IRF8-expressing CD123Trends in immunology · 2023Review
- Loss of IRF8 inhibits the growth of acute myeloid leukemia cells.Annals of hematology · 2023Article
- Article
- Tumor-associated macrophages expressing the transcription factor IRF8 promote T cell exhaustion in cancer.Immunity · 2022Article
- Review
- IRF8 Impacts Self-Renewal of Hematopoietic Stem Cells by Regulating TLR9 Signaling Pathway of Innate Immune Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Multimodal Intralesional Therapy for Reshaping the Myeloid Compartment of Tumors Resistant to Anti-PD-L1 Therapy via IRF8 Expression.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Developmental pathways of myeloid-derived suppressor cells in neoplasia.Cellular immunology · 2021Review
- Autocrine IL6-Mediated Activation of the STAT3-DNMT Axis Silences the TNFα-RIP1 Necroptosis Pathway to Sustain Survival and Accumulation of Myeloid-Derived Suppressor Cells.Cancer research · 2020Article
- Tissue-resident ductal macrophages survey the mammary epithelium and facilitate tissue remodelling.Nature cell biology · 2020Article
- Cohesin Members Stag1 and Stag2 Display Distinct Roles in Chromatin Accessibility and Topological Control of HSC Self-Renewal and Differentiation.Cell stem cell · 2019Article
- Identification of monocyte-like precursors of granulocytes in cancer as a mechanism for accumulation of PMN-MDSCs.The Journal of experimental medicine · 2019Article
- Transcription factors IRF8 and PU.1 are required for follicular B cell development and BCL6-driven germinal center responses.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Epigenetic control of early dendritic cell lineage specification by the transcription factor IRF8 in mice.Blood · 2019Article
- Haemopedia RNA-seq: a database of gene expression during haematopoiesis in mice and humans.Nucleic acids research · 2019Article
- T follicular helper cells restricted by IRF8 contribute to T cell-mediated inflammation.Journal of autoimmunity · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
13 authors.
Funding
Abstract
The IFN regulatory factor family member 8 (IRF8) regulates differentiation of lymphoid and myeloid lineage cells by promoting or suppressing lineage-specific genes. How IRF8 promotes hematopoietic progenitors to commit to one lineage while preventing the development of alternative lineages is not known. In this study, we report an IRF8-EGFP fusion protein reporter mouse that revealed previously unrecognized patterns of IRF8 expression. Differentiation of hematopoietic stem cells into oligopotent progenitors is associated with progressive increases in IRF8-EGFP expression. However, significant induction of IRF8-EGFP is found in granulocyte-myeloid progenitors and the common lymphoid progenitors but not the megakaryocytic-erythroid progenitors. Surprisingly, IRF8-EGFP identifies three subsets of the seemingly homogeneous granulocyte-myeloid progenitors with an intermediate level of expression of EGFP defining bipotent progenitors that differentiation into either EGFP(hi) monocytic progenitors or EGFP(lo) granulocytic progenitors. Also surprisingly, IRF8-EGFP revealed a highly heterogeneous pre-pro-B population with a fluorescence intensity ranging from background to 4 orders above background. Interestingly, IRF8-EGFP readily distinguishes true B cell committed (EGFP(int)) from those that are noncommitted. Moreover, dendritic cell progenitors expressed extremely high levels of IRF8-EGFP. Taken together, the IRF8-EGFP reporter revealed previously unrecognized subsets with distinct developmental potentials in phenotypically well-defined oligopotent progenitors, providing new insights into the dynamic heterogeneity of developing hematopoietic progenitors.
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