ArticleProceedings of the National Academy of Sciences of the United States of America2014
Functional genomics of Lactobacillus casei establishment in the gut.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
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- Resistance to host antimicrobial peptides mediates resilience of gut commensals during infection and aging inProceedings of the National Academy of Sciences of the United States of America · 2023Article
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- In Vitro Probiotic Characterization and Safety Assessment of Lactic Acid Bacteria Isolated from Raw Milk of Japanese-Saanen Goat (Animals : an open access journal from MDPI · 2022Article
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- A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Production and Characterization of High Value Prebiotics From Biorefinery-Relevant Feedstocks.Frontiers in microbiology · 2021Article
- Study of theFrontiers in microbiology · 2020Article
- Experimental bacterial adaptation to the zebrafish gut reveals a primary role for immigration.PLoS biology · 2018Article
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- New Genes Involved in Mild Stress Response Identified by Transposon Mutagenesis inFrontiers in microbiology · 2018Article
- D-Alanylation of teichoic acids contributes to Lactobacillus plantarum-mediated Drosophila growth during chronic undernutrition.Nature microbiology · 2017Article
- The essential genomic landscape of the commensal Bifidobacterium breve UCC2003.Scientific reports · 2017Article
- From homeostasis to pathology: decrypting microbe-host symbiotic signals in the intestinal crypt.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Dysbiosis of small intestinal microbiota in liver cirrhosis and its association with etiology.Scientific reports · 2016Article
- Draft Genome Sequence of Lactobacillus casei Lbs2.Genome announcements · 2014Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Although the composition of the gut microbiota and its symbiotic contribution to key host physiological functions are well established, little is known as yet about the bacterial factors that account for this symbiosis. We selected Lactobacillus casei as a model microorganism to proceed to genomewide identification of the functions required for a symbiont to establish colonization in the gut. As a result of our recent development of a transposon-mutagenesis tool that overcomes the barrier that had prevented L. casei random mutagenesis, we developed a signature-tagged mutagenesis approach combining whole-genome reverse genetics using a set of tagged transposons and in vivo screening using the rabbit ligated ileal loop model. After sequencing transposon insertion sites in 9,250 random mutants, we assembled a library of 1,110 independent mutants, all disrupted in a different gene, that provides a representative view of the L. casei genome. By determining the relative quantity of each of the 1,110 mutants before and after the in vivo challenge, we identified a core of 47 L. casei genes necessary for its establishment in the gut. They are involved in housekeeping functions, metabolism (sugar, amino acids), cell wall biogenesis, and adaptation to environment. Hence we provide what is, to our knowledge, the first global functional genomics analysis of L. casei symbiosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.