ArticlePlanta2014
Cloning and characterization of a novel fructan 6-exohydrolase strongly inhibited by sucrose in Lolium perenne.
Article in Planta, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Variation of Fructan and Its Metabolizing Enzymes in Onions with Different Storage Characteristics.Journal of applied glycoscience · 2025Article
- The genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms.BMC plant biology · 2023Article
- Review
- A Fructan Exohydrolase from Maize Degrades Both Inulin and Levan and Co-Exists with 1-Kestotriose in Maize.International journal of molecular sciences · 2021Article
- A 6&1-FEH Encodes an Enzyme for Fructan Degradation and Interact with Invertase Inhibitor Protein in Maize (International journal of molecular sciences · 2019Article
- Novel fructan exohydrolase: unique properties and applications for human health.Journal of experimental botany · 2018Article
- Short-term effects of defoliation intensity on sugar remobilization and N fluxes in ryegrass.Journal of experimental botany · 2018Article
- Chicory R2R3-MYB transcription factors CiMYB5 and CiMYB3 regulate fructan 1-exohydrolase expression in response to abiotic stress and hormonal cues.Journal of experimental botany · 2017Article
- Depletion of carbohydrate reserves limits nitrate uptake during early regrowth in Lolium perenne L.Journal of experimental botany · 2017Article
- Exogenous Classic Phytohormones Have Limited Regulatory Effects on Fructan and Primary Carbohydrate Metabolism in Perennial Ryegrass (Lolium perenne L.).Frontiers in plant science · 2015Article
- Effects of different carbohydrate sources on fructan metabolism in plants of Chrysolaena obovata grown in vitro.Frontiers in plant science · 2015Article
- A Simple and Fast Kinetic Assay for the Determination of Fructan Exohydrolase Activity in Perennial Ryegrass (Lolium perenne L.).Frontiers in plant science · 2015Article
- Does gibberellin biosynthesis play a critical role in the growth of Lolium perenne? Evidence from a transcriptional analysis of gibberellin and carbohydrate metabolic genes after defoliation.Frontiers in plant science · 2015Article
- Fructan synthesis, accumulation and polymer traits. II. Fructan pools in populations of perennial ryegrass (Lolium perenne L.) with variation for water-soluble carbohydrate and candidate genes were not correlated with biosynthetic activity and demonstrated constraints to polymer chain extension.Frontiers in plant science · 2015Article
- Spatiotemporal Dynamics of Oligofructan Metabolism and Suggested Functions in Developing Cereal Grains.Frontiers in plant science · 2015Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionThe first 6-fructan exohydrolase (6-FEH) cDNA from Lolium perenne was cloned and characterized. Following defoliation, Lp6 - FEHa transcript level unexpectedly decreased together with an increase in total FEH activity. Lolium perenne is a major forage grass species that accumulates fructans, mainly composed of β(2,6)-linked fructose units. Fructans are mobilized through strongly increased activities of fructan exohydrolases (FEHs), sustaining regrowth following defoliation. To understand the complex regulation of fructan breakdown in defoliated grassland species, the objective was to clone and characterize new FEH genes in L. perenne. To find FEH genes related to refoliation, a defoliated tiller base cDNA library was screened. Characterization of the recombinant protein was performed in Pichia pastoris. In this report, the cloning and enzymatic characterization of the first 6-FEH from L. perenne is described. Following defoliation, during fructan breakdown, Lp6-FEHa transcript level unexpectedly decreased in elongating leaf bases (ELB) and in mature leaf sheaths (tiller base) in parallel to increased total FEH activities. In comparison, transcript levels of genes coding for fructosyltransferases (FTs) involved in fructan biosynthesis also decreased after defoliation but much faster than FEH transcript levels. Since Lp6-FEHa was strongly inhibited by sucrose, mechanisms modulating FEH activities are discussed. It is proposed that differences in the regulation of FEH activity among forage grasses influence their tolerance to defoliation.
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