ArticleUltrasound in medicine & biology2014
Non-contact high-frequency ultrasound microbeam stimulation for studying mechanotransduction in human umbilical vein endothelial cells.
Article in Ultrasound in medicine & biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- HIFU-triggered burst release of gallic acid from gelatin/polyvinyl pyrrolidone hydrogel network crosslinked with magnesium gallate MOF.Ultrasonics sonochemistry · 2026Article
- Tumour priming by ultrasound mechanogenetics for CAR T therapy.Nature materials · 2026Article
- Recent Advancements in High-Frequency Ultrasound Applications from Imaging to Microbeam Stimulation.Sensors (Basel, Switzerland) · 2024Review
- Automated cell-type classification combining dilated convolutional neural networks with label-free acoustic sensing.Scientific reports · 2022Article
- Biological Effects and Applications of Bulk and Surface Acoustic Waves on In Vitro Cultured Mammal Cells: New Insights.Biomedicines · 2022Review
- Automated estimation of cancer cell deformability with machine learning and acoustic trapping.Scientific reports · 2022Article
- The application of mechanobiotechnology for immuno-engineering and cancer immunotherapy.Frontiers in cell and developmental biology · 2022Review
- Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach.Applied physics letters · 2021Article
- Sensitization of nerve cells to ultrasound stimulation through Piezo1-targeted microbubbles.Ultrasonics sonochemistry · 2021Article
- Cytosolic CaTheranostics · 2021Article
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- Activation of Piezo1 but Not NaUltrasound in medicine & biology · 2018Article
- Low-Intensity Ultrasound Modulates CaAnnals of biomedical engineering · 2018Article
- Biomechanical Characterization at the Cell Scale: Present and Prospects.Frontiers in physiology · 2018Review
- Multifunctional single beam acoustic tweezer for non-invasive cell/organism manipulation and tissue imaging.Scientific reports · 2016Article
- Direct and sustained intracellular delivery of exogenous molecules using acoustic-transfection with high frequency ultrasound.Scientific reports · 2016Article
- Impedance matching network for high frequency ultrasonic transducer for cellular applications.Ultrasonics · 2016Article
- Acoustic tweezers for studying intracellular calcium signaling in SKBR-3 human breast cancer cells.Ultrasonics · 2015Article
- A feasibility study ofApplied physics letters · 2014Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca(2+)) elevation in human umbilical vein endothelial cells. The cellular mechanotransduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. A variety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca(2+) levels. It was found that the response was dose dependent, and moreover, extracellular Ca(2+) and cytoplasmic Ca(2+) stores were involved in the Ca(2+) elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified.
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