ArticleBMC musculoskeletal disorders2014
Dose-dependent regulation of cell proliferation and collagen degradation by estradiol on ligamentum flavum.
Article in BMC musculoskeletal disorders, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 34 citations in OpenAlex.
- Impact of Menopause and Associated Hormonal Changes on Spine Health in Older Females: A Review.Cells · 2026Review
- miR-29b-3p Affects the Hypertrophy of Ligamentum Flavum in Lumbar Spinal Stenosis and its Mechanism.Biochemical genetics · 2025Article
- Editorial: Women in science: musculoskeletal pain.Frontiers in pain research (Lausanne, Switzerland) · 2025Article
- Risk Factors for Surgical Treatment of Lumbar Degenerative Disc Disease in Middle-aged and Older Women: A Prospective Case-Control Study of 2370 Subjects.Orthopaedic surgery · 2024Article
- Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy.Molecular medicine reports · 2022Article
- Expression of Estrogen Receptor Alpha and Evaluation of Histological Degeneration Scores in Fibroblasts of Hypertrophied Ligamentum Flavum: A Qualitative Study.Biomolecules · 2021Article
- Integrative analysis of genome-wide DNA methylation and single-nucleotide polymorphism identified ACSM5 as a suppressor of lumbar ligamentum flavum hypertrophy.Arthritis research & therapy · 2021Article
- The Difference in Gender Affects the Pathogenesis of Ligamentum Flavum Hypertrophy.Spine surgery and related research · 2018Article
- Article
- Effect of Estrogen on Musculoskeletal Performance and Injury Risk.Frontiers in physiology · 2018Review
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEstradiol plays an important role in the regulation of collagen metabolism. Deficiency of estradiol has been reported to be associated with the degeneration of many connective tissues. However, the association of estradiol and hypertrophy of the ligamentum flavum was seldom explored. Therefore, we studied the effects of estradiol on cultured cells from the ligamentum flavum.
methodsPrimary cultures of human ligamentum flavum cells obtained from surgical specimens of 14 patients undergoing spinal surgery were used to investigate the effect of estradiol on cell proliferation and the expression of collagen, elastin, and matrix metalloproteinases. Downstream pathways of estrogen receptor underlying the regulation of metalloproteinases were also investigated.
resultsIn our study, we revealed the existence of estrogen receptors on both female and male ligamentum flavum cells with a gender difference. 17β-estradiol increased early (24 hours) proliferation of ligamentum flavum cells in a dose dependent manner and the effect could not be seen when the cell density increased. Estradiol with a concentration of 10(-9) M decreased collagen levels and increased the expression of MMP-13. Adding an antagonist of PI3K downstream pathway could reverse the expression of MMP-13 caused by estradiol.
conclusionsThe results implied estradiol regulated the expression of MMP-13 via PI3K pathway and contributed to the homeostasis of extracellular matrix in the ligamentum flavum.
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