ArticleUrolithiasis2014
Increased oxidative DNA damage seen in renal biopsies adjacent stones in patients with nephrolithiasis.
Article in Urolithiasis, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 28 citations in OpenAlex.
- Myricetin Attenuates Ethylene Glycol-Induced Nephrolithiasis in Rats via Mitigating Oxidative Stress and Inflammatory Markers.Applied biochemistry and biotechnology · 2024Article
- A prospective case-control study on the evaluation of oxidative stress in renal stone formers.Urolithiasis · 2024Article
- The effect ofNorthern clinics of Istanbul · 2024Article
- A novel immune-related model to predict prognosis and responsiveness to checkpoint and angiogenesis blockade therapy in advanced renal cancer.Frontiers in oncology · 2023Article
- Calcium oxalate crystals trigger epithelial-mesenchymal transition and carcinogenic features in renal cells: a crossroad between kidney stone disease and renal cancer.Experimental hematology & oncology · 2022Article
- HydroZitLa inhibits calcium oxalate stone formation in nephrolithic rats and promotes longevity in nematode Caenorhabditis elegans.Scientific reports · 2022Article
- New Insights into the Pathogenesis of Diabetic Nephropathy: Proximal Renal Tubules Are Primary Target of Oxidative Stress in Diabetic Kidney.Acta histochemica et cytochemica · 2020Article
- Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells.Scientific reports · 2019Article
- Vitamin D and calcium kidney stones: a review and a proposal.International urology and nephrology · 2019Review
- The effects of HAP and macrophage cells to the expression of inflammatory factors and apoptosis in HK-2 cells of vitro co-cultured system.Urolithiasis · 2018Article
- Do kidney stone formers have a kidney disease?Kidney international · 2015Review
- In vitro anti-lithogenic activity of lime powder regimen (LPR) and the effect of LPR on urinary risk factors for kidney stone formation in healthy volunteers.Urolithiasis · 2015Article
- Evaluation of change in oxidative stress in urolithiasis patients following complete stone clearance: A prospective observational study.Indian journal of urology : IJU : journal of the Urological Society of IndiaArticle
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8 authors at 2 institutions in 1 country.
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Abstract
Urinary excretion of 8-hydroxydeoxyguanosine (8-OHdG), a marker of oxidative DNA damage, is significantly higher in nephrolithiasis patients than in healthy individuals, indicating that these patients have higher degree of oxidative stress. In the present study, we investigated 8-OHdG expression in renal biopsies of patients with nephrolithiasis and in renal tubular cells (HK-2 cells) exposed to calcium oxalate monohydrate (COM). We performed immunohistochemical staining for 8-OHdG in renal biopsies adjacent stones obtained from 28 patients with nephrolithiasis. Controls were noncancerous renal tissues from nephrectomies of patients with renal cancer. 8-OHdG was overexpressed in the nucleus of renal tubular cells in patients with nephrolithiasis compared with controls. Only one nephrolithiasis biopsy was negative for 8-OHdG, whereas in 19 cases 8-OHdG was highly expressed. The level of expression of 8-OHdG among patients with calcium oxalate (mostly mixed with calcium phosphate) and uric acid stones was not significantly different. Increased leukocyte infiltration was observed in renal tissues from patients with nephrolithiasis. Exposure of HK-2 cells to COM caused increased intracellular reactive oxygen species and nuclear expression of 8-OHdG. To our knowledge, this is the first report of increased 8-OHdG expression in renal tubular cells of patients with nephrolithiasis. In vitro, COM crystals were capable of inducing oxidative damage of DNA in the proximal renal tubular cells.
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