Evidence map›Paper›PMID 25020062›Full record

ArticlePloS one2014

Expansion of human mesenchymal stromal cells from fresh bone marrow in a 3D scaffold-based system under direct perfusion.

Adam Papadimitropoulos, Elia Piccinini, Sophie Brachat, Alessandra Braccini, David Wendt, Andrea Barbero, Carsten Jacobi, Ivan Martin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Adam PapadimitropoulosDepartments of Surgery and of Biomedicine, Institute for Surgical Research and Hospital Management, University Hospital Basel, University of Basel, Basel, Switzerland.
Elia PiccininiDepartments of Surgery and of Biomedicine, Institute for Surgical Research and Hospital Management, University Hospital Basel, University of Basel, Basel, Switzerland.
Sophie BrachatMusculoSkeletal Diseases, Novartis Institutes for Biomedical Research, Basel, Switzerland.
Alessandra BracciniDepartments of Surgery and of Biomedicine, Institute for Surgical Research and Hospital Management, University Hospital Basel, University of Basel, Basel, Switzerland.
David WendtDepartments of Surgery and of Biomedicine, Institute for Surgical Research and Hospital Management, University Hospital Basel, University of Basel, Basel, Switzerland.
Andrea BarberoDepartments of Surgery and of Biomedicine, Institute for Surgical Research and Hospital Management, University Hospital Basel, University of Basel, Basel, Switzerland.
Carsten JacobiMusculoSkeletal Diseases, Novartis Institutes for Biomedical Research, Basel, Switzerland.
Ivan MartinDepartments of Surgery and of Biomedicine, Institute for Surgical Research and Hospital Management, University Hospital Basel, University of Basel, Basel, Switzerland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stromal/stem cell (MSC) expansion in conventional monolayer culture on plastic dishes (2D) leads to progressive loss of functionality and thus challenges fundamental studies on the physiology of skeletal progenitors, as well as translational applications for cellular therapy and molecular medicine. Here we demonstrate that 2D MSC expansion can be entirely bypassed by culturing freshly isolated bone marrow nucleated cells within 3D porous scaffolds in a perfusion-based bioreactor system. The 3D-perfusion system generated a stromal tissue that could be enzymatically treated to yield CD45- MSC. As compared to 2D-expanded MSC (control), those derived from 3D-perfusion culture after the same time (3 weeks) or a similar extent of proliferation (7-8 doublings) better maintained their progenitor properties, as assessed by a 4.3-fold higher clonogenicity and the superior differentiation capacity towards all typical mesenchymal lineages. Transcriptomic analysis of MSC from 5 donors validated the robustness of the process and indicated a reduced inter-donor variability and a significant upregulation of multipotency-related gene clusters following 3D-perfusion--as compared to 2D-expansion. Interestingly, the differences in functionality and transcriptomics between MSC expanded in 2D or under 3D-perfusion were only partially captured by cytofluorimetric analysis using conventional surface markers. The described system offers a multidisciplinary approach to study how factors of a 3D engineered niche regulate MSC function and, by streamlining conventional labor-intensive processes, is prone to automation and scalability within closed bioreactor systems.

Indexed as

BioreactorsCell Culture TechniquesCell ProliferationCell SeparationHumansMesenchymal Stem CellsOligonucleotide Array Sequence AnalysisPerfusionPhenotype

Identifiers

PMID25020062
PMCPMC4096512

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.