ArticlePloS one2014
Molecular detection of Chlamydia trachomatis and other sexually transmitted bacteria in semen of male partners of infertile couples in Tunisia: the effect on semen parameters and spermatozoa apoptosis markers.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 74 citations in OpenAlex.
- Serum IgG1 and IgG3 Antibodies to Chlamydia trachomatis Pgp3 and Hsp60 in Men of Subfertile Couples.The Journal of infectious diseases · 2026Article
- Mechanisms and applications of mesenchymal stem cell exosomes in enhancing fertility.Stem cell research & therapy · 2025Review
- Article
- Sexually Transmitted Infections: Usefulness of Molecular Methods for Microorganism Detection in Stored Sexual Assault Samples.International journal of molecular sciences · 2025Article
- Causal association of the skin microbiome with human infertility: insights from a bidirectional two-sample Mendelian randomization.Archives of dermatological research · 2025Article
- Research progress on the mechanism underlying the application of mesenchymal stem cells in the treatment of male infertility.Frontiers in endocrinology · 2025Review
- HPV and co-infections: impacts on semen inflammation, oxidative stress, and sperm quality.Frontiers in cellular and infection microbiology · 2025Article
- Effect of Postbiotics Derived fromNutrients · 2024Article
- Leukocytospermia and/or Bacteriospermia: Impact on Male Infertility.Journal of clinical medicine · 2024Review
- Unraveling the Intricacies of the Seminal Microbiome and Its Impact on Human Fertility.Biology · 2024Review
- Effect of environmental factors on seminal microbiome and impact on sperm quality.Frontiers in endocrinology · 2024Review
- Prevalence ofEthiopian journal of health sciences · 2023Article
- Genetic Polymorphisms in MicroRNA-196a2 and the Risk of Human Abortion Related to Mycoplasma hominis.Current microbiology · 2022Article
- Bacteriospermia - A formidable player in male subfertility.Open life sciences · 2022Review
- Role of Infection and Leukocytes in Male Infertility.Advances in experimental medicine and biology · 2022Article
- Beneficial Effects of Lactic Acid Bacteria on Animal Reproduction Function.Veterinary medicine international · 2022Review
- Using the PCR and Blood Agar in Diagnosis of Semen Bacterial Contamination of Fertile and Infertile Men.Reports of biochemistry & molecular biology · 2021Article
- Article
- Bacteriospermia and its association with seminal fluid parameters and infertility in infertile men, Kerman, Iran: A cross-sectional study.International journal of reproductive biomedicine · 2021Article
- Mesenchymal Stem-Cell Derived Exosome Therapy as a Potential Future Approach for Treatment of Male Infertility Caused byFrontiers in microbiology · 2021Review
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was undertaken to determine the prevalence of Chlamydia trachomatis, Mycoplasmas, and Ureaplasmas in semen samples of the male partners of infertile couples and to investigate whether Chlamydia trachomatis could initiate apoptosis in human spermatozoa. A total of 85 males partners of infertile couples undergoing routine semen analysis according to World Health Organization guidelines were included. Specimens were examined for the presence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum and Ureaplasma parvum by Real time PCR (qPCR). Semen specimens were analysed for the appearance of apoptotic markers (sperm DNA fragmentation, activated caspase 3 levels, mitochondrial membrane potential (ΔΨm)) using flow cytometry. C. trachomatis, N. gonorrhoeae, U. urealyticum, M genitalium were detected in semen samples of 13 (15.2%), 5 (5.8%), 5 (5.8%) and 3 (3.5%) male partners of infertile couples, respectively. M. hominis and U. parvum were detected in semen sample of only one patient (1.1%). The semen of infertile men positive for C. trachomatis showed lower mean of semen count and lower rapid progressive motility (category [a]) of spermatozoa compared to uninfected men with statistically significances (p = 0.02 and p = 0.04, respectively). Flow cytometry analyses demonstrated a significant increase of the mean rate of semen with low ΔΨm and caspase 3 activation of infertile men positive for C. trachomatis compared to uninfected men (p = 0.006 and p = 0.001, respectively). DNA fragmentation was also increased in sperm of infertile men positive for C. trachomatis compared to uninfected men but without statistical significances (p = 0.62). Chlamydial infection was associated to loss of ΔΨm and caspase 3activation. Thus, C. trachomatis infection could be incriminated in apoptosis induction of spermatozoa. These effects may explain the negative direct impact of C. trachomatis infection on sperm fertilizing ability.
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