ArticlePloS one2014
Zinc regulates meiotic resumption in porcine oocytes via a protein kinase C-related pathway.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Nivalenol affects Cyclin B1 level and activates SAC for cell cycle progression in mouse oocyte meiosis.Cell proliferation · 2022Article
- Role of zinc in female reproduction.Biology of reproduction · 2021Review
- Meiosis Resumption of Immature Human Oocytes following Treatment with Calcium IonophoreCell journal · 2021Article
- Bovine eggs release zinc in response to parthenogenetic and sperm-induced egg activation.Theriogenology · 2019Article
- Zinc: A Necessary Ion for Mammalian Sperm Fertilization Competency.International journal of molecular sciences · 2018Review
- Zinc sparks induce physiochemical changes in the egg zona pellucida that prevent polyspermy.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- MLL2 is essential for porcine embryo development in vitro.In vitro cellular & developmental biology. Animal · 2016Article
- Pig oocyte activation using a Zn²⁺ chelator, TPEN.Theriogenology · 2015Article
- The inorganic anatomy of the mammalian preimplantation embryo and the requirement of zinc during the first mitotic divisions.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Article
- Article
- Fe(III) Is Essential for Porcine Embryonic Development via Mitochondrial Function Maintenance.PloS one · 2015Article
- Article
Corrections and comments
- Expression of concern · 2019-08-13Concerns/Issues about Data · Concerns/Issues about Image · Original Data and/or Images not Provided and/or not Available ·
- Expression of concern
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zinc is an extremely important trace element that plays important roles in several biological processes. However, the function of zinc in meiotic division of porcine oocytes is unknown. In this study, we investigated the role of zinc during meiotic resumption in in vitro matured porcine oocytes. During meiotic division, a massive release of zinc was observed. The level of free zinc in the cytoplasm significantly increased during maturation. Depletion of zinc using N, N, N', N'-tetrakis (2-pyridylmethyl) ethylenediamine (TPEN), a Zn2+ chelator, blocked meiotic resumption in a dose dependent manner. The level of phosphorylated mitogen activated protein kinase (MAPK) and p34cdc2 kinase activity were reduced when zinc was depleted. Moreover, zinc depletion reduced the levels of phosphorylated protein kinase C (PKC) substrates in a dose dependent manner. Real-time PCR analysis showed that expression of the MAPK- and maturation promoting factor related genes C-mos, CyclinB1, and Cdc2 was downregulated following zinc depletion. Treatment with the PKC agonist phorbol 12-myristate 13-acetate (PMA) increased phosphorylation of PKC substrates and MAPK and increased p34cdc2 kinase activity. This rescued the meiotic arrest, even in the presence of TPEN. Activation of PKC by PMA increased the level of zinc in the cytoplasm. These data demonstrate that zinc is required for meiotic resumption in porcine oocytes, and this appears to be regulated via a PKC related pathway.
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