ArticlePloS one2014
Annexin5 plays a vital role in Arabidopsis pollen development via Ca2+-dependent membrane trafficking.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
- Genome-Wide Identification and Characterization of the Annexin (ANN) Gene Family in Potato (Genes · 2026Article
- Peanut annexin AhANN6 promotes heat resistance in plant and bacterial cells.Plant molecular biology · 2025Article
- Rab5 GTPases mediate the targeting of ROP signalling to establish polarity for pollen germination.Nature plants · 2025Article
- The evolution, variation and expression patterns of the annexin gene family in the maize pan-genome.Scientific reports · 2025Article
- Annexins: central regulators of plant growth and stress signaling.Acta biochimica et biophysica Sinica · 2025Review
- The rice annexin genePlant direct · 2023Article
- High temporal-resolution transcriptome landscapes of maize embryo sac and ovule during early seed development.Plant molecular biology · 2023Article
- ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution inPlants (Basel, Switzerland) · 2023Article
- Article
- Genome-Wide Characterization and Abiotic Stresses Expression Analysis of Annexin Family Genes in Poplar.International journal of molecular sciences · 2022Article
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- Lectin receptor-like kinase LecRK-VIII.2 is a missing link in MAPK signaling-mediated yield control.Plant physiology · 2021Article
- Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes inInternational journal of molecular sciences · 2021Article
- The Tip-Localized Phosphatidylserine Established by Arabidopsis ALA3 Is Crucial for Rab GTPase-Mediated Vesicle Trafficking and Pollen Tube Growth.The Plant cell · 2020Article
- Comprehensive analyses of the annexin (ANN) gene family in Brassica rapa, Brassica oleracea and Brassica napus reveals their roles in stress response.Scientific reports · 2020Article
- Mechanics of Pollen Tube Elongation: A Perspective.Frontiers in plant science · 2020Review
- Article
- Secretory Vesicles Targeted to Plasma Membrane During Pollen Germination and Tube Growth.Frontiers in cell and developmental biology · 2020Review
- Flower bud proteome reveals modulation of sex-biased proteins potentially associated with sex expression and modification in dioecious Coccinia grandis.BMC plant biology · 2019Article
- Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulation of pollen development and pollen tube growth is a complicated biological process that is crucial for sexual reproduction in flowering plants. Annexins are widely distributed from protists to higher eukaryotes and play multiple roles in numerous cellular events by acting as a putative "linker" between Ca2+ signaling, the actin cytoskeleton and the membrane, which are required for pollen development and pollen tube growth. Our recent report suggested that downregulation of the function of Arabidopsis annexin 5 (Ann5) in transgenic Ann5-RNAi lines caused severely sterile pollen grains. However, little is known about the underlying mechanisms of the function of Ann5 in pollen. This study demonstrated that Ann5 associates with phospholipid membrane and this association is stimulated by Ca2+ in vitro. Brefeldin A (BFA) interferes with endomembrane trafficking and inhibits pollen germination and pollen tube growth. Both pollen germination and pollen tube growth of Ann5-overexpressing plants showed increased resistance to BFA treatment, and this effect was regulated by calcium. Overexpression of Ann5 promoted Ca2+-dependent cytoplasmic streaming in pollen tubes in vivo in response to BFA. Lactrunculin (LatB) significantly prohibited pollen germination and tube growth by binding with high affinity to monomeric actin and preferentially targeting dynamic actin filament arrays and preventing actin polymerization. Overexpression of Ann5 did not affect pollen germination or pollen tube growth in response to LatB compared with wild-type, although Ann5 interacts with actin filaments in a manner similar to some animal annexins. In addition, the sterile pollen phenotype could be only partially rescued by Ann5 mutants at Ca2+-binding sites when compared to the complete recovery by wild-type Ann5. These data demonstrated that Ann5 is involved in pollen development, germination and pollen tube growth through the promotion of endomembrane trafficking modulated by calcium. Our results provide reliable molecular mechanisms that underlie the function of Ann5 in pollen.
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