ArticleJournal of clinical & experimental ophthalmology2013
Stressor-dependent Alterations in Glycoprotein 130: Implications for Glial Cell Reactivity, Cytokine Signaling and Ganglion Cell Health in Glaucoma.
Article in Journal of clinical & experimental ophthalmology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma.Frontiers in neuroscience · 2019Article
- Impact of Graphene on the Efficacy of Neuron Culture Substrates.Advanced healthcare materials · 2018Article
- Ccl5 Mediates Proper Wiring of Feedforward and Lateral Inhibition Pathways in the Inner Retina.Frontiers in neuroscience · 2018Article
- Constitutive and Stress-induced Expression of CCL5 Machinery in Rodent Retina.Journal of clinical & cellular immunology · 2017Article
- Hyper-IL-6: a potent and efficacious stimulator of RGC regeneration.Eye (London, England) · 2017Review
- Interleukin-6 Deficiency Attenuates Retinal Ganglion Cell Axonopathy and Glaucoma-Related Vision Loss.Frontiers in neuroscience · 2017Article
- Boosting Central Nervous System Axon Regeneration by Circumventing Limitations of Natural Cytokine Signaling.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Interleukin-6: A Constitutive Modulator of Glycoprotein 130, Neuroinflammatory and Cell Survival Signaling in Retina.Journal of clinical & cellular immunology · 2016Article
- Primate neural retina upregulates IL-6 and IL-10 in response to a herpes simplex vector suggesting the presence of a pro-/anti-inflammatory axis.Experimental eye research · 2016Article
- Retina-on-a-chip: a microfluidic platform for point access signaling studies.Biomedical microdevices · 2015Article
- Pressure-Induced Alterations in PEDF and PEDF-R Expression: Implications for Neuroprotective Signaling in Glaucoma.Journal of clinical & experimental ophthalmology · 2015Article
- Early pro-inflammatory cytokine elevations in the DBA/2J mouse model of glaucoma.Journal of neuroinflammation · 2015Article
- Astrocyte Reactivity: A Biomarker for Retinal Ganglion Cell Health in Retinal Neurodegeneration.Journal of clinical & cellular immunology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
objectiveThe interleukin-6 (IL-6) family of cytokines is associated with retinal ganglion cell (RGC) survival and glial reactivity in glaucoma. The purpose of this study was to evaluate glaucoma-related changes in glycoprotein-130 (gp130), the common signal transducer of the IL-6 family of cytokines, as they relate to RGC health, glial reactivity and expression of IL-6 cytokine family members.
methodsFor all experiments, we examined healthy retina (young C57), aged retina (aged C57), retina predisposed to glaucoma (young DBA/2) and retina with IOP-induced glaucoma (aged DBA/2). We determined retinal gene expression of gp130 and IL-6 family members, using quantitative PCR, and protein expression of gp130, using multiplex ELISA. For protein localization and cell-specific expression, we performed co-immunolabeling for gp130 and cell type-specific markers. We used quantitative microscopy to measure layer-specific expression of gp130 and its relationships to astrocyte and Müller glia reactivity and RGC axonal transport, as determined by uptake and transport of cholera toxin β-subunit (CTB).
resultsGene expression of gp130 was elevated with all glaucoma-related stressors, but only normal aging increased protein levels. In healthy retina, gp130 localized primarily to the inner retina, where it was expressed by astrocytes, Müller cells and RGCs. Layer-specific analysis of gp130 expression revealed increased expression in aging retina and decreased expression in glaucomatous retina that was eccentricity-dependent. These glaucoma-related changes in gp130 expression correlated with the level of GFAP and glutamine synthetase expression, as well as axonal transport in RGCs. The relationships between gp130, glial reactivity and RGC health could impact signaling by many IL-6 family cytokines, which exhibited overall increased expression in a stressor-dependent manner.
conclusionsGlaucoma-related stressors, including normal aging, glaucoma predisposition and IOP-induced glaucoma, differentially alter expression of gp130 and these alterations have direct implications for astrocyte and Müller glia reactivity, RGC health and cytokine signaling.
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