Evidence map›Paper›PMID 25018881›Full record

ArticleInternational journal of proteomics2014

Prediction of spontaneous regression of cervical intraepithelial neoplasia lesions grades 2 and 3 by proteomic analysis.

Kai-Erik Uleberg, Irene Tveiterås Ovestad, Ane Cecilie Munk, Cato Brede, Bianca van Diermen, Einar Gudlaugsson, Emiel A M Janssen, Anne Hjelle, Jan P A Baak

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of proteomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Article
  4. Review
  5. Cancer Immunoprevention: Current Status and Future Directions.Archivum immunologiae et therapiae experimentalis · 2021
    Review
  6. Article
  7. Review
  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Kai-Erik UlebergNorconsult AS, Section 355 QA Service, P.O. Box 216, NO-4503 Mandal, Norway ; Former International Research Institute of Stavanger (IRIS), P.O. Box 8046, 4068 Stavanger, Norway.
Irene Tveiterås OvestadPathology Department, Stavanger University Hospital, P.O. Box 8100, 4068 Stavanger, Norway.
Ane Cecilie MunkDepartment of Gynecology and Obstetrics, Stavanger University Hospital, P.O. Box 8100, 4068 Stavanger, Norway.
Cato BredeDepartment of Medical Biochemistry, Stavanger University Hospital, P.O. Box 8100, 4068 Stavanger, Norway.ORCID 0000-0003-2691-6052
Bianca van DiermenPathology Department, Stavanger University Hospital, P.O. Box 8100, 4068 Stavanger, Norway.
Einar GudlaugssonPathology Department, Stavanger University Hospital, P.O. Box 8100, 4068 Stavanger, Norway.
Emiel A M JanssenPathology Department, Stavanger University Hospital, P.O. Box 8100, 4068 Stavanger, Norway.
Anne HjelleFormer International Research Institute of Stavanger (IRIS), P.O. Box 8046, 4068 Stavanger, Norway ; Mediteam AS, Sjøveien 34, 4315 Sandnes, Norway.
Jan P A BaakPathology Department, Stavanger University Hospital, P.O. Box 8100, 4068 Stavanger, Norway ; The Gade Institute, University of Bergen, P.O. Box 1400, 5021 Bergen, Norway.
Stavanger University Hospital · NOInternational Research Institute of Stavanger · NONorconsult (Norway) · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Regression of cervical intraepithelial neoplasia (CIN) 2-3 to CIN 1 or less is associated with immune response as demonstrated by immunohistochemistry in formaldehyde-fixed paraffin-embedded (FFPE) biopsies. Proteomic analysis of water-soluble proteins in supernatants of biopsy samples with LC-MS (LTQ-Orbitrap) was used to identify proteins predictive of CIN2-3 lesions regression. CIN2-3 in the biopsies and persistence (CIN2-3) or regression (≤CIN1) in follow-up cone biopsies was validated histologically by two experienced pathologists. In a learning set of 20 CIN2-3 (10 regressions and 10 persistence cases), supernatants were depleted of seven high abundance proteins prior to unidimensional LC-MS/MS protein analysis. Mean protein concentration was 0.81 mg/mL (range: 0.55-1.14). Multivariate statistical methods were used to identify proteins that were able to discriminate between regressive and persistent CIN2-3. The findings were validated in an independent test set of 20 CIN2-3 (10 regressions and 10 persistence cases). Multistep identification criteria identified 165 proteins. In the learning set, zinc finger protein 441 and phospholipase D6 independently discriminated between regressive and persistent CIN2-3 lesions and correctly classified all 20 patients. Nine regression and all persistence cases were correctly classified in the validation set. Zinc finger protein 441 and phospholipase D6 in supernatant samples detected by LTQ-Orbitrap can predict regression of CIN2-3.

Identifiers

PMID25018881
PMCPMC4082862
OpenAlexW2056283989

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.