ArticleNucleic acids research2014
Formation of circular polyribosomes on eukaryotic mRNA without cap-structure and poly(A)-tail: a cryo electron tomography study.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Circularization and Ribosome Recycling: From Polysome Topology to Translational Control.International journal of molecular sciences · 2026Review
- Polyribosomes of circular topology are prevalent in mammalian cells.Nucleic acids research · 2023Article
- A transformation clustering algorithm and its application in polyribosomes structural profiling.Nucleic acids research · 2022Article
- mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation.Nucleic acids research · 2022Article
- Circular RNA regulates the onset and progression of cancer through the mitogen-activated protein kinase signaling pathway.Oncology letters · 2021Review
- The LARP1 La-Module recognizes both ends of TOP mRNAs.RNA biology · 2021Article
- Controversies around the function of LARP1.RNA biology · 2021Review
- Functional Cyclization of Eukaryotic mRNAs.International journal of molecular sciences · 2020Article
- Revisiting the Closed-Loop Model and the Nature of mRNA 5'-3' Communication.Molecular cell · 2018Review
- mRNP architecture in translating and stress conditions reveals an ordered pathway of mRNP compaction.The Journal of cell biology · 2018Article
- ZNF598 Is a Quality Control Sensor of Collided Ribosomes.Molecular cell · 2018Article
- Circular RNAs: Regulators of Cancer-Related Signaling Pathways and Potential Diagnostic Biomarkers for Human Cancers.Theranostics · 2017Review
- Non-canonical Translation in Plant RNA Viruses.Frontiers in plant science · 2017Review
- Inter-polysomal coupling of termination and initiation during translation in eukaryotic cell-free system.Scientific reports · 2016Article
- Peering at Brain Polysomes with Atomic Force Microscopy.Journal of visualized experiments : JoVE · 2016Article
- Linking Α to Ω: diverse and dynamic RNA-based mechanisms to regulate gene expression by 5'-to-3' communication.F1000Research · 2016Review
- RiboAbacus: a model trained on polyribosome images predicts ribosome density and translational efficiency from mammalian transcriptomes.Nucleic acids research · 2015Article
- Three distinct ribosome assemblies modulated by translation are the building blocks of polysomes.The Journal of cell biology · 2015Article
- Mechanism of cytoplasmic mRNA translation.The arabidopsis book · 2015Article
- Conformation transitions of eukaryotic polyribosomes during multi-round translation.Nucleic acids research · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polyribosomes newly formed on recombinant GFP-encoding mRNAs in a wheat germ cell-free translation system were analyzed using cryo-electron tomography, with sub-tomogram averaging of polysomal ribosomes and reconstruction of 3D structures of individual polyribosomes. The achieved level of resolution in the reconstructed polyribosomes allowed deducing the mRNA path by connecting adjacent exit and entry sites at the ribosomes inside each polyribosome. In this way, the circularity of a significant fraction (about 50%) of translating polyribosomes was proved in the case of the capped poly(A)-tailed mRNA, in agreement with the existing paradigm of the circularization via interaction of cap-bound initiation factor eIF4F with poly(A)-binding protein. However, translation of the capped mRNA construct without poly(A) tail, but with unspecific 3'-UTR derived from non-coding plasmid sequence, also led to the formation of circular polyribosomes in similar proportion (40%). Moreover, the polyribosomes formed on the uncapped non-polyadenylated mRNA with non-synergistic 5'- and 3'-UTRs proved to be circular as well, and appeared in the same proportion as in the previous cases. Thus, the formation of circular polyribosomes was found to be virtually independent of the presence of cap structure and poly(A) tail in mRNA, in contrast to the longstanding paradigm in the field.
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