ArticleJournal of biomechanics2014
The influence of substrate elastic modulus on retinal pigment epithelial cell phagocytosis.
Article in Journal of biomechanics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 0 citations in OpenAlex.
- Functional scaffolds design strategies for retinal repair and regeneration.Materials today. Bio · 2025Review
- Influence of Substrate Stiffness on iPSC-Derived Retinal Pigmented Epithelial Cells.Stem cells translational medicine · 2024Article
- The impact of substrate stiffness on morphological, transcriptional and functional aspects in RPE.Scientific reports · 2024Article
- Progress in Stem Cells-Based Replacement Therapy for Retinal Pigment Epithelium: In Vitro Differentiation to In Vivo Delivery.Stem cells translational medicine · 2023Review
- Fabrication and Evaluation of Gellan Gum/Hyaluronic Acid Hydrogel for Retinal Tissue Engineering Biomaterial and the Influence of Substrate Stress Relaxation on Retinal Pigment Epithelial Cells.Molecules (Basel, Switzerland) · 2022Article
- Coculture techniques for modeling retinal development and disease, and enabling regenerative medicine.Stem cells translational medicine · 2020Review
- Utilizing Recombinant Spider Silk Proteins To Develop a Synthetic Bruch's Membrane for Modeling the Retinal Pigment Epithelium.ACS biomaterials science & engineering · 2019Article
- Effects of bevacizumab, ranibizumab, and aflibercept on phagocytic properties in human RPE cybrids with AMD versus normal mitochondria.Experimental eye research · 2018Article
- Activin A improves retinal pigment epithelial cell survival on stiff but not soft substrates.Journal of biomedical materials research. Part A · 2018Article
- The formation of a functional retinal pigment epithelium occurs on porous polytetrafluoroethylene substrates independently of the surface chemistry.Journal of materials science. Materials in medicine · 2017Article
- The influence of substrate modulus on retinal pigment epithelial cells.Journal of biomedical materials research. Part A · 2017Article
- A Step by Step Protocol for Subretinal Surgery in Rabbits.Journal of visualized experiments : JoVE · 2016Article
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
To better understand if a complex process such as phagocytosis is influenced by substrate stiffness, we investigated the influence of substrate elastic modulus on phagocytosis in the retinal pigment epithelial (RPE) cell line ARPE-19. RPE cells lie on Bruch's membrane, directly under the retina, and phagocytose the shed photoreceptor outer segments. Bruch's membrane is known to increase in stiffness by an order of magnitude with age and thus, this study has potential relevance in explaining retinal changes in age-related macular degeneration. ARPE-19 cells were plated on laminin-coated polyacrylamide substrates of varying elastic modulus. After 14 days in culture, a solution of latex fluorescent beads suspended in PBS was placed in each well. After an incubation time of 4h, flow cytometry was performed to determine the number of cells that phagocytosed a bead. The number of ARPE-19 cells that phagocytosed a bead decreased continuously as a function of increasing substrate elastic modulus (p=0.0135), and this was found to be a linear relationship (slope=-0.03305 ± 0.01104, R2=0.4726 per 10,000 cells). Our results suggest that RPE cells display decreased phagocytosis when grown on firmer substrates, and thus, RPE cells in older eyes, in which Bruch's membrane is stiffer, may demonstrate decreased phagocytosis. Impaired phagocytosis by RPE cells may contribute to impaired metabolism of photoreceptor outer segments and to development of macular degeneration. Material stiffness may be a critical parameter in the development of neural therapies, including retinal prosthetics and stem cell therapies.
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Registered trials
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