ArticleInvestigative ophthalmology & visual science2014
Notch signaling functions in retinal pericyte survival.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The Calcium Signalling Profile of the Inner Blood-Retinal Barrier in Diabetic Retinopathy.Cells · 2025Review
- Pericytes: The lung-forgotten cell type.Frontiers in physiology · 2023Review
- Notch Signaling in Vascular Endothelial and Mural Cell Communications.Cold Spring Harbor perspectives in medicine · 2022Article
- Notch3 Signaling and Aggregation as Targets for the Treatment of CADASIL and Other NOTCH3-Associated Small-Vessel Diseases.The American journal of pathology · 2021Review
- Deficiency of Notch signaling in pericytes results in arteriovenous malformations.JCI insight · 2020Article
- Pericyte-Endothelial Interactions in the Retinal Microvasculature.International journal of molecular sciences · 2020Review
- A Pilot Study on MicroRNA Profile in Tear Fluid to Predict Response to Anti-VEGF Treatments for Diabetic Macular Edema.Journal of clinical medicine · 2020Article
- NOTCH1 signaling induces pathological vascular permeability in diabetic retinopathy.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Roles of Pericytes in Stroke Pathogenesis.Cell transplantation · 2018Review
- Vascular stem/progenitor cells: functions and signaling pathways.Cellular and molecular life sciences : CMLS · 2018Review
- Evaluation of Notch3 Deficiency in Diabetes-Induced Pericyte Loss in the Retina.Journal of vascular research · 2018Article
- Asymmetry at cell-cell interfaces direct cell sorting, boundary formation, and tissue morphogenesis.Experimental cell research · 2017Review
- Vascular Pericyte Impairment and Connexin43 Gap Junction Deficit Contribute to Vasomotor Decline in Diabetic Retinopathy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Therapeutic antibody targeting of Notch3 signaling prevents mural cell loss in CADASIL.The Journal of experimental medicine · 2017Article
- Notch Signaling in Vascular Smooth Muscle Cells.Advances in pharmacology (San Diego, Calif.) · 2017Review
- Coculture Assays for Endothelial Cells-Mural Cells Interactions.Methods in molecular biology (Clifton, N.J.) · 2016Article
- SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes.Journal of ophthalmology · 2016Article
- Combined deficiency of Notch1 and Notch3 causes pericyte dysfunction, models CADASIL, and results in arteriovenous malformations.Scientific reports · 2015Article
- Immunolocalization of platelet-derived growth factor receptor-β (PDGFR-β) and pericytes in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).Neuropathology and applied neurobiology · 2015Article
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Authors and funding
6 authors.
Funding
Abstract
purposePericytes, the vascular cells that constitute the outer layer of capillaries, have been shown to have a crucial role in vascular development and stability. Loss of pericytes precedes endothelial cell dysfunction and vascular degeneration in small-vessel diseases, including diabetic retinopathy. Despite their clinical relevance, the cellular pathways controlling survival of retinal pericytes remain largely uncharacterized. Therefore, we investigated the role of Notch signaling, a master regulator of cell fate decisions, in retinal pericyte survival.
methodsA coculture system of ligand-dependent Notch signaling was developed using primary cultured retinal pericytes and a mesenchymal cell line derived from an inducible mouse model expressing the Delta-like 1 Notch ligand. This model was used to examine the effect of Notch activity on pericyte survival using quantitative PCR (qPCR) and a light-induced cell death assay. The effect of Notch gain- and loss-of-function was analyzed in monocultures of retinal pericytes using antibody arrays to interrogate the expression of apoptosis-related proteins.
resultsPrimary cultured retinal pericytes differentially expressed key molecules of the Notch pathway and displayed strong expression of canonical Notch/RBPJK (recombination signal-binding protein 1 for J-kappa) downstream targets. A gene expression screen using gain- and loss-of-function approaches identified genes relevant to cell survival as downstream targets of Notch activity in retinal pericytes. Ligand-mediated Notch activity protected retinal pericytes from light-induced cell death.
conclusionsOur results have identified signature genes downstream of Notch activity in retinal pericytes and suggest that tight regulation of Notch signaling is crucial for pericyte survival.
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