ArticleBMC genomics2014
De novo sequencing and comparative analysis of holy and sweet basil transcriptomes.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 129 citations in OpenAlex.
- Article
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- Supplementary UV-A and UV-B radiation differentially regulate morphology in Ocimum basilicum.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Identification of Key Aromatic Compounds in Basil (Metabolites · 2023Article
- Overexpression of Terpenoid Biosynthesis Genes Modifies Root Growth and Nodulation in Soybean (Cells · 2022Article
- Challenges in Medicinal and Aromatic Plants DNA Barcoding-Lessons from the Lamiaceae.Plants (Basel, Switzerland) · 2022Review
- Virus-Induced Gene Silencing for Functional Genomics of Specialized Metabolism in Medicinal Plants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A glance at the chemodiversity ofPhytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2022Review
- Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss.Scientific reports · 2021Article
- Emerging roles of NAC transcription factor in medicinal plants: progress and prospects.3 Biotech · 2021Review
- The interactive effect of aromatic amino acid composition on the accumulation of phenolic compounds and the expression of biosynthesis-related genes inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Review
- Overexpression of Terpenoid Biosynthesis Genes From Garden Sage (Frontiers in plant science · 2021Article
- The genome sequence of tetraploid sweet basil, Ocimum basilicum L., provides tools for advanced genome editing and molecular breeding.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Article
- Anthocyanins from3 Biotech · 2020Article
- Comparative transcriptome analysis to identify putative genes related to trichome development in Ocimum species.Molecular biology reports · 2020Article
- Genome sequencing of four culinary herbs reveals terpenoid genes underlying chemodiversity in the Nepetoideae.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOcimum L. of family Lamiaceae is a well known genus for its ethnobotanical, medicinal and aromatic properties, which are attributed to innumerable phenylpropanoid and terpenoid compounds produced by the plant. To enrich genomic resources for understanding various pathways, de novo transcriptome sequencing of two important species, O. sanctum and O. basilicum, was carried out by Illumina paired-end sequencing.
resultsThe sequence assembly resulted in 69117 and 130043 transcripts with an average length of 1646 ± 1210.1 bp and 1363 ± 1139.3 bp for O. sanctum and O. basilicum, respectively. Out of the total transcripts, 59648 (86.30%) and 105470 (81.10%) from O. sanctum and O. basilicum, and respectively were annotated by uniprot blastx against Arabidopsis, rice and lamiaceae. KEGG analysis identified 501 and 952 transcripts from O. sanctum and O. basilicum, respectively, related to secondary metabolism with higher percentage of transcripts for biosynthesis of terpenoids in O. sanctum and phenylpropanoids in O. basilicum. Higher digital gene expression in O. basilicum was validated through qPCR and correlated to higher essential oil content and chromosome number (O. sanctum, 2n = 16; and O. basilicum, 2n = 48). Several CYP450 (26) and TF (40) families were identified having probable roles in primary and secondary metabolism. Also SSR and SNP markers were identified in the transcriptomes of both species with many SSRs linked to phenylpropanoid and terpenoid pathway genes.
conclusionThis is the first report of a comparative transcriptome analysis of Ocimum species and can be utilized to characterize genes related to secondary metabolism, their regulation, and breeding special chemotypes with unique essential oil composition in Ocimum.
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