ArticleJournal of cell science2014
Divergent and convergent roles for kinases and phosphatases in neurofilament dynamics.
Article in Journal of cell science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Neurofilament Biophysics: From Structure to Biomechanics.Molecular biology of the cell · 2024Review
- Neurofilament light chain: a biomarker at the crossroads of clarity and confusion for gene-directed therapies.Neurodegenerative disease management · 2024Review
- Mutations in protein kinase Cγ promote spinocerebellar ataxia type 14 by impairing kinase autoinhibition.Science signaling · 2022Article
- Regulation of Axon Initial Segment Diameter by COUP-TFI Fine-tunes Action Potential Generation.Neuroscience bulletin · 2022Article
- Vasculocentric Axonal NfH in Small Vessel Disease.Journal of neuropathology and experimental neurology · 2022Article
- Article
- Neurofilaments: neurobiological foundations for biomarker applications.Brain : a journal of neurology · 2020Review
- Axonal transport and neurological disease.Nature reviews. Neurology · 2019Review
- The sympathetic nervous system regulates skeletal muscle motor innervation and acetylcholine receptor stability.Acta physiologica (Oxford, England) · 2019Article
- Pharmacophore-based models for therapeutic drugs against phosphorylated tau in Alzheimer's disease.Drug discovery today · 2019Review
- Advances in the Potential Biomarkers of Epilepsy.Frontiers in neurology · 2019Review
- Cytoskeleton as a Target of Quinolinic Acid Neurotoxicity: Insight from Animal Models.Molecular neurobiology · 2018Review
- Assembly and turnover of neurofilaments in growing axonal neurites.Biology open · 2018Article
- Influence of a GSK3β phosphorylation site within the proximal C-terminus of Neurofilament-H on neurofilament dynamics.Biology open · 2017Article
- Neurobiology of axonal transport defects in motor neuron diseases: Opportunities for translational research?Neurobiology of disease · 2017Review
- Catalpol promotes cellular apoptosis in human HCT116 colorectal cancer cells via microRNA-200 and the downregulation of PI3K-Akt signaling pathway.Oncology letters · 2017Article
- Phosphorylation-Induced Mechanical Regulation of Intrinsically Disordered Neurofilament Proteins.Biophysical journal · 2017Article
- Preclinical and Clinical Studies for Sodium Tungstate: Application in Humans.Journal of clinical & cellular immunology · 2015Article
- The high molecular weight neurofilament subunit plays an essential role in axonal outgrowth and stabilization.Biology open · 2014Article
- Diffuse axonal injury in brain trauma: insights from alterations in neurofilaments.Frontiers in cellular neuroscience · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
C-terminal neurofilament phosphorylation mediates cation-dependent self-association leading to neurofilament incorporation into the stationary axonal cytoskeleton. Multiple kinases phosphorylate the C-terminal domains of the heavy neurofilament subunit (NF-H), including cyclin-dependent protein kinase 5 (CDK5), mitogen-activated protein kinases (MAPKs), casein kinase 1 and 2 (CK1 and CK2) and glycogen synthase kinase 3β (GSK3β). The respective contributions of these kinases have been confounded because they phosphorylate multiple substrates in addition to neurofilaments and display extensive interaction. Herein, differentiated NB2a/d1 cells were transfected with constructs expressing GFP-tagged NF-H, isolated NF-H sidearms and NF-H lacking the distal-most 187 amino acids. Cultures were treated with roscovitine, PD98059, Li(+), D4476, tetrabromobenzotriazole and calyculin, which are active against CDK5, MKK1 (also known as MAP2K1), GSK3β, CK1, CK2 and protein phosphatase 1 (PP1), respectively. Sequential phosphorylation by CDK5 and GSK3β mediated the neurofilament-neurofilament associations. The MAPK pathway (i.e. MKK1 to ERK1/2) was found to downregulate GSK3β, and CK1 activated PP1, both of which promoted axonal transport and restricted neurofilament-neurofilament associations to axonal neurites. The MAPK pathway and CDK5, but not CK1 and GSK3β, inhibited neurofilament proteolysis. These findings indicate that phosphorylation of neurofilaments by the proline-directed MAPK pathway and CDK5 counterbalance the impact of phosphorylation of neurofilaments by the non-proline-directed CK1 and GSK3β.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.