ArticleThe Biochemical journal2014
Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine.
Article in The Biochemical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- Comparative Genomic and Genetic Evidence on a Role for the OarX Protein in Thiamin Salvage.ACS omega · 2024Article
- Article
- A Possible Aquatic Origin of the Thiaminase TenA of the Human Gut Symbiont Bacteroides thetaiotaomicron.Journal of molecular evolution · 2023Article
- B vitamin supply in plants and humans: the importance of vitamer homeostasis.The Plant journal : for cell and molecular biology · 2022Review
- Natural Variation in Vitamin BFrontiers in plant science · 2022Article
- Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.Plant physiology · 2021Article
- Metabolic engineering of rice endosperm towards higher vitamin B1 accumulation.Plant biotechnology journal · 2021Article
- The importance of thiamine (vitamin BThe Journal of biological chemistry · 2020Review
- Thiaminase I Provides a Growth Advantage by Salvaging Precursors from Environmental Thiamine and Its Analogs in Burkholderia thailandensis.Applied and environmental microbiology · 2018Article
- The PLUTO plastidial nucleobase transporter also transports the thiamin precursor hydroxymethylpyrimidine.Bioscience reports · 2018Article
- Article
- A Novel Transcriptional Regulator Related to Thiamine Phosphate Synthase Controls Thiamine Metabolism Genes in Archaea.Journal of bacteriology · 2017Article
- Does Abiotic Stress Cause Functional B Vitamin Deficiency in Plants?Plant physiology · 2016Review
- Arabidopsis TH2 Encodes the Orphan Enzyme Thiamin Monophosphate Phosphatase.The Plant cell · 2016Article
- Divisions of labor in the thiamin biosynthetic pathway among organs of maize.Frontiers in plant science · 2014Article
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
Abstract
The TenA protein family occurs in prokaryotes, plants and fungi; it has two subfamilies, one (TenA_C) having an active-site cysteine, the other (TenA_E) not. TenA_C proteins participate in thiamin salvage by hydrolysing the thiamin breakdown product amino-HMP (4-amino-5-aminomethyl-2-methylpyrimidine) to HMP (4-amino-5-hydroxymethyl-2-methylpyrimidine); the function of TenA_E proteins is unknown. Comparative analysis of prokaryote and plant genomes predicted that (i) TenA_E has a salvage role similar to, but not identical with, that of TenA_C and (ii) that TenA_E and TenA_C also have non-salvage roles since they occur in organisms that cannot make thiamin. Recombinant Arabidopsis and maize TenA_E proteins (At3g16990, GRMZM2G080501) hydrolysed amino-HMP to HMP and, far more actively, hydrolysed the N-formyl derivative of amino-HMP to amino-HMP. Ablating the At3g16990 gene in a line with a null mutation in the HMP biosynthesis gene ThiC prevented its rescue by amino-HMP. Ablating At3g16990 in the wild-type increased sensitivity to paraquat-induced oxidative stress; HMP overcame this increased sensitivity. Furthermore, the expression of TenA_E and ThiC genes in Arabidopsis and maize was inversely correlated. These results indicate that TenA_E proteins mediate amidohydrolase and aminohydrolase steps in the salvage of thiamin breakdown products. As such products can be toxic, TenA_E proteins may also pre-empt toxicity.
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