ArticlePloS one2014
Analysis of the functional interaction of Arabidopsis starch synthase and branching enzyme isoforms reveals that the cooperative action of SSI and BEs results in glucans with polymodal chain length distribution similar to amylopectin.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 44 citations in OpenAlex.
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- Soluble and insoluble α-glucan synthesis in yeast by enzyme suites derived exclusively from maize endosperm.Plant physiology · 2023Article
- Transcriptomic analysis of mesocarp tissue during fruit development of the oil palm revealed specific isozymes related to starch metabolism that control oil yield.Frontiers in plant science · 2023Article
- A Review of Starch Biosynthesis in Relation to the Building Block-Backbone Model.International journal of molecular sciences · 2020Review
- Starch and Glycogen Analyses: Methods and Techniques.Biomolecules · 2020Review
- Theoretical and experimental approaches to understand the biosynthesis of starch granules in a physiological context.Photosynthesis research · 2020Article
- Article
- Starch formation inside plastids of higher plants.Protoplasma · 2018Review
- Analyses of starch biosynthetic protein complexes and starch properties from developing mutant rice seeds with minimal starch synthase activities.BMC plant biology · 2018Article
- Parameters of Starch Granule Genesis in Chloroplasts ofFrontiers in plant science · 2018Review
- Association mapping of starch chain length distribution and amylose content in pea (Pisum sativum L.) using carbohydrate metabolism candidate genes.BMC plant biology · 2017Article
- Differences in specificity and compensatory functions among three major starch synthases determine the structure of amylopectin in rice endosperm.Plant molecular biology · 2017Article
- Starch Synthase 4 and Plastidal Phosphorylase Differentially Affect Starch Granule Number and Morphology.Plant physiology · 2017Article
- Formation of starch in plant cells.Cellular and molecular life sciences : CMLS · 2016Review
- Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
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Abstract
Starch synthase (SS) and branching enzyme (BE) establish the two glycosidic linkages existing in starch. Both enzymes exist as several isoforms. Enzymes derived from several species were studied extensively both in vivo and in vitro over the last years, however, analyses of a functional interaction of SS and BE isoforms are missing so far. Here, we present data from in vitro studies including both interaction of leaf derived and heterologously expressed SS and BE isoforms. We found that SSI activity in native PAGE without addition of glucans was dependent on at least one of the two BE isoforms active in Arabidopsis leaves. This interaction is most likely not based on a physical association of the enzymes, as demonstrated by immunodetection and native PAGE mobility analysis of SSI, BE2, and BE3. The glucans formed by the action of SSI/BEs were analysed using leaf protein extracts from wild type and be single mutants (Atbe2 and Atbe3 mutant lines) and by different combinations of recombinant proteins. Chain length distribution (CLD) patterns of the formed glucans were irrespective of SSI and BE isoforms origin and still independent of assay conditions. Furthermore, we show that all SS isoforms (SSI-SSIV) were able to interact with BEs and form branched glucans. However, only SSI/BEs generated a polymodal distribution of glucans which was similar to CLD pattern detected in amylopectin of Arabidopsis leaf starch. We discuss the impact of the SSI/BEs interplay for the CLD pattern of amylopectin.
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