ArticleBiochimie2014
Sphingolipids and ceramides of mouse aqueous humor: Comparative profiles from normotensive and hypertensive DBA/2J mice.
Article in Biochimie, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Lipids and lipid regulators in intraocular pressure homeostasis.Current opinion in pharmacology · 2025Review
- Analysis of Sphingosine and Sphinganine from the Aqueous Humor for Signaling Studies Using Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Optimized Lipidomics Extraction of Sphingosine and Sphinganine from Optic Nerve for Signaling Studies.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Identification of the novel role of sterol regulatory element binding proteins (SREBPs) in mechanotransduction and intraocular pressure regulation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- An overview of lipidomics utilizing cadaver derived biological samples.Expert review of proteomics · 2021Article
- Differential metabolic markers associated with primary open-angle glaucoma and cataract in human aqueous humor.BMC ophthalmology · 2020Article
- Differentiation of soluble aqueous humor metabolites in primary open angle glaucoma and controls.Experimental eye research · 2020Article
- Endogenous ocular lipids as potential modulators of intraocular pressure.Journal of cellular and molecular medicine · 2020Article
- The Role of Deimination in Regenerative Reprogramming of Neurons.Molecular neurobiology · 2019Article
- Analysis of sphingolipid composition in human vitreous from control and diabetic individuals.Journal of diabetes and its complications · 2019Article
- Role of Bioactive Sphingolipids in Inflammation and Eye Diseases.Advances in experimental medicine and biology · 2019Article
- Metabolomics and Age-Related Macular Degeneration.Metabolites · 2018Review
- Shotgun Sphingolipid Analysis of Human Aqueous Humor.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy.Journal of cellular biochemistry · 2017Article
- Thermally labile components of aqueous humor potently induce osteogenic potential in adipose-derived mesenchymal stem cells.Experimental eye research · 2015Article
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Authors and funding
4 authors.
Funding
Abstract
purposeTo identify the sphingolipid and ceramide species and their quantitative differences between normotensive and hypertensive intraocular pressure states in DBA/2J mouse aqueous humor (AH).
methodsNormotensive and hypertensive AH was sampled from mice by paracentesis. Lipid extraction was performed using modifications of the Bligh and Dyer method. Protein concentration was estimated using the Bradford colorimetric assay. Sphingolipids and ceramides were identified and subjected to ratiometric quantification using appropriate class specific lipid standards on a TSQ Quantum Access Max triple quadrupole mass spectrometer.
resultsThe comparative profiles of normotensive and hypertensive DBA/2J mouse AH showed several species of sphingomyelin, sphingoid base, sphingoid base-1-phosphate (S1P) and ceramides common between them. A number of unique lipids in each of the above lipid classes were also identified in normotensive AH that were absent in hypertensive AH and vice versa.
conclusionA number of sphingolipid and ceramide species were found to be uniquely present in normotensive, but absent in hypertensive AH and vice versa. Further pursuit of these findings is likely to contribute towards expanding our understanding of the molecular changes associated with increased intraocular pressure (IOP) and glaucoma.
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