ArticleToxins2014
Identification and characterization of the insecticidal toxin "makes caterpillars floppy" in Photorhabdus temperata M1021 using a cosmid library.
Article in Toxins, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Biological control of Schistocerca gregaria and Locusta migratoria migratorioides using Entomopathogenic bacteria.Scientific reports · 2025Article
- Genome Sequence Analysis of NativeToxins · 2024Article
- Functional Comparison of Three Chitinases from Symbiotic Bacteria of Entomopathogenic Nematodes.Toxins · 2024Article
- Genetic Engineering ofAccess microbiology · 2023Article
- Development of a cost-effective medium for Photorhabdus temperata bioinsecticide production from wastewater and exploration of performance kinetic.Scientific reports · 2021Article
- The differential strain virulence of the candidate toxins of3 Biotech · 2020Article
- Comparison of Xenorhabdus bovienii bacterial strain genomes reveals diversity in symbiotic functions.BMC genomics · 2015Article
- Induced autoprocessing of the cytopathic Makes caterpillars floppy-like effector domain of the Vibrio vulnificus MARTX toxin.Cellular microbiology · 2015Article
- Evolutionary patchwork of an insecticidal toxin shared between plant-associated pseudomonads and the insect pathogens Photorhabdus and Xenorhabdus.BMC genomics · 2015Article
- Benzaldehyde as an insecticidal, antimicrobial, and antioxidant compound produced by Photorhabdus temperata M1021.Journal of microbiology (Seoul, Korea) · 2015Article
- An insecticidal compound produced by an insect-pathogenic bacterium suppresses host defenses through phenoloxidase inhibition.Molecules (Basel, Switzerland) · 2014Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photorhabdus temperata is an entomopathogenic enterobacterium; it is a nematode symbiont that possesses pathogenicity islands involved in insect virulence. Herein, we constructed a P. temperata M1021 cosmid library in Escherichia coli XL1-Blue MRF` and obtained 7.14 × 105 clones. However, only 1020 physiologically active clones were screened for insect virulence factors by injection of each E. coli cosmid clone into Galleria mellonella and Tenebrio molitor larvae. A single cosmid clone, PtC1015, was consequently selected due to its characteristic virulent properties, e.g., loss of body turgor followed by death of larvae when the clone was injected into the hemocoel. The sequence alignment against the available sequences in Swiss-Prot and NCBI databases, confirmed the presence of the mcf gene homolog in the genome of P. temperata M1021 showing 85% homology and 98% query coverage with the P. luminescens counterpart. Furthermore, a 2932 amino acid long Mcf protein revealed limited similarity with three protein domains. The N-terminus of the Mcf encompassed consensus sequence for a BH3 domain, the central region revealed similarity to toxin B, and the C-terminus of Mcf revealed similarity to the bacterial export domain of ApxIVA, an RTX-like toxin. In short, the Mcf toxin is likely to play a role in the elimination of insect pests, making it a promising model for use in the agricultural field.
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Registered trials
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