ArticleCornea2014
UV cross-linking of donor corneas confers resistance to keratolysis.
Article in Cornea, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- How Modifications of Corneal Cross-Linking Protocols Influence Corneal Resistance to Enzymatic Digestion and Treatment Depth.Translational vision science & technology · 2023Article
- Donor Cornea Use in Scleral Surface ReconstructionTurkish journal of ophthalmology · 2021Article
- Penetrating keratoplasty using collagen crosslinked donor tissue: A case report.American journal of ophthalmology case reports · 2021Article
- Double-masked, sham and placebo-controlled trial of corneal cross-linking and topical difluprednate in the treatment of bacterial keratitis: Steroids and Cross-linking for Ulcer Treatment Trial (SCUT II) study protocol.BMJ open ophthalmology · 2021Article
- Enzymatic Digestion of Porcine Corneas Cross-linked by Hypo- and Hyperosmolar Formulations of Riboflavin/ultraviolet A or WST11/Near-Infrared Light.Translational vision science & technology · 2020Article
- A review of long-term corneal preservation techniques: Relevance and renewed interests in the COVID-19 era.Indian journal of ophthalmology · 2020Review
- Matrix Metalloproteinase-Deactivating Contact Lens for Corneal Melting.ACS biomaterials science & engineering · 2019Article
- Long-term clinical outcomes of keratoplasty using gamma-irradiated corneal lenticules.BMJ open ophthalmology · 2019Article
- Article
- Corneal inflammation after miniature keratoprosthesis implantation.Investigative ophthalmology & visual science · 2014Article
- Collagen cross-linking of the Boston keratoprosthesis donor carrier to prevent corneal melting in high-risk patients.Eye & contact lens · 2014Review
- Glaucoma Patch Graft Surgery Utilizing Corneas Augmented with Collagen Cross-linking.Middle East African journal of ophthalmologyArticle
- Clinical Efficacy of Ex Vivo Cross-Linking on Neovascularization of the Donor Cornea Carrier Tissue for the Boston Type I Keratoprosthesis.Ophthalmology scienceArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
purposeThe aim of this study was to develop a modified ex vivo corneal cross-linking method that increases stromal resistance to enzymatic degradation for use as a carrier for the Boston keratoprosthesis.
methodsEx vivo cross-linking of human corneas was performed using Barron artificial anterior chambers. The corneas were deepithelialized, pretreated with riboflavin solution (0.1% riboflavin/20% dextran), and irradiated with ultraviolet A (UV-A) light (λ = 370 nm, irradiance = 3 mW/cm) for various durations. The combined effect of UV-A and gamma (γ) irradiation was also assessed using the commercially available γ-irradiated corneal donors. The corneas were then trephined and incubated at 37°C with 0.3% collagenase A solution. The time to dissolution of each cornea was compared across treatments.
resultsDeepithelialized corneas (no UV light, no riboflavin) dissolved in 5.8 ± 0.6 hours. Cross-linked corneas demonstrated increased resistance to dissolution, with a time to dissolution of 17.8 ± 2.6 hours (P < 0.0001). The corneal tissues' resistance to collagenase increased with longer UV-A exposure, reaching a plateau at 30 minutes. Cross-linking both the anterior and posterior corneas did not provide added resistance when compared with cross-linking the anterior corneas only (P > 0.05). γ-irradiated corneas dissolved as readily as deepithelialized controls regardless of whether they were further cross-linked (5.6 ± 1.2 hours) or not (6.1 ± 0.6 hours) (P = 0.43).
conclusionsCollagen cross-linking of the deepithelialized anterior corneal surface for 30 minutes conferred optimal resistance to in vitro keratolysis by collagenase A.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.