ArticlePloS one2014
Collection and chemical composition of phloem sap from Citrus sinensis L. Osbeck (sweet orange).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 182 citations in OpenAlex.
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- A Citrus medica stem cutting-based platform for rapid screening of therapeutic compounds against Candidatus Liberibacter asiaticus.Scientific reports · 2026Article
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- Galleria mellonella possesses the essential nutritional needs to host the fastidious Huanglongbing bacterial pathogen 'Candidatus Liberibacter asiaticus'.Communications biology · 2025Article
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- Evergreen citrus trees exhibit distinct seasonal nitrogen remobilization patterns between mature leaves and bark.Horticulture research · 2025Article
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- Fertilization Strategies in Huanglongbing-InfectedPlants (Basel, Switzerland) · 2025Article
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- Histochemical, metabolic and ultrastructural changes in leaf patelliform nectaries explain extrafloral nectar synthesis and secretion in Clerodendrum chinense.Annals of botany · 2024Article
- Evolutionary and Structural Analysis of PP16 in Viridiplantae.International journal of molecular sciences · 2024Article
- Integrated metabolomic and transcriptomic analyses reveal the molecular mechanism of amino acid transport between source and sink during tea shoot development.Plant cell reports · 2024Article
- Long-term nitrogen fertilization alters the partitioning of amino acids between citrus leaves and fruits.Frontiers in plant science · 2024Article
- Gamma-Aminobutyric Acid Supplementation Boosts the Phytohormonal Profile in 'Plants (Basel, Switzerland) · 2023Article
- Symbioses shape feeding niches and diversification across insects.Nature ecology & evolution · 2023Article
- Phloem Sap Composition: What Have We Learnt from Metabolomics?International journal of molecular sciences · 2023Review
- Quick Decline and Stem PittingPlants (Basel, Switzerland) · 2023Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Through utilizing the nutrient-rich phloem sap, sap feeding insects such as psyllids, leafhoppers, and aphids can transmit many phloem-restricted pathogens. On the other hand, multiplication of phloem-limited, uncultivated bacteria such as Candidatus Liberibacter asiaticus (CLas) inside the phloem of citrus indicates that the sap contains all the essential nutrients needed for the pathogen growth. The phloem sap composition of many plants has been studied; however, to our knowledge, there is no available data about citrus phloem sap. In this study, we identified and quantified the chemical components of phloem sap from pineapple sweet orange. Two approaches (EDTA enhanced exudation and centrifugation) were used to collect phloem sap. The collected sap was derivatized with methyl chloroformate (MCF), N-methyl-N- [tert-butyl dimethylsilyl]-trifluroacetamide (MTBSTFA), or trimethylsilyl (TMS) and analyzed with GC-MS revealing 20 amino acids and 8 sugars. Proline, the most abundant amino acid, composed more than 60% of the total amino acids. Tryptophan, tyrosine, leucine, isoleucine, and valine, which are considered essential for phloem sap-sucking insects, were also detected. Sucrose, glucose, fructose, and inositol were the most predominant sugars. In addition, seven organic acids including succinic, fumaric, malic, maleic, threonic, citric, and quinic were detected. All compounds detected in the EDTA-enhanced exudate were also detected in the pure phloem sap using centrifugation. The centrifugation technique allowed estimating the concentration of metabolites. This information expands our knowledge about the nutrition requirement for citrus phloem-limited bacterial pathogen and their vectors, and can help define suitable artificial media to culture them.
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