ArticlePloS one2014
Reliable quantification of protein expression and cellular localization in histological sections.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- IL-8 blockade enhances anti-PD-1 therapeutic activity in renal cell carcinoma in a next-generation patient-derived xenograft model.iScience · 2026Article
- STAT1/3/5 Functions Driving Lipid and Energy Metabolism in Cancer and Immunity.International journal of molecular sciences · 2026Review
- Thyroid hormone receptor beta signaling is a targetable driver of prostate cancer growth.Molecular cancer · 2025Article
- STAT3/LKB1 controls metastatic prostate cancer by regulating mTORC1/CREB pathway.Molecular cancer · 2023Article
- Growth hormone and nonalcoholic fatty liver disease.Immunometabolism (Cobham, Surrey) · 2023 · on this mapReview
- Thyroid and androgen receptor signaling are antagonized by μ-Crystallin in prostate cancer.International journal of cancer · 2021Article
- p16 in highly malignant esophageal carcinomas: the correlation with clinicopathological factors and human papillomavirus infection.Virchows Archiv : an international journal of pathology · 2021Article
- Digital Microscopy, Image Analysis, and Virtual Slide Repository.ILAR journal · 2018Article
- SOX2 and Rb1 in esophageal small-cell carcinoma: their possible involvement in pathogenesis.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2017Article
- Adipocyte STAT5 deficiency promotes adiposity and impairs lipid mobilisation in mice.Diabetologia · 2017Article
- Increased survival and cell cycle progression pathways are required for EWS/FLI1-induced malignant transformation.Cell death & disease · 2016Article
- Localization of TrkB and p75 receptors in peritoneal and deep infiltrating endometriosis: an immunohistochemical study.Reproductive biology and endocrinology : RB&E · 2016Article
- Mouse tissue distribution and persistence of the food-born fusariotoxins Enniatin B and Beauvericin.Toxicology letters · 2016Article
- Cooperation of ETV6/RUNX1 and BCL2 enhances immunoglobulin production and accelerates glomerulonephritis in transgenic mice.Oncotarget · 2016Article
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- Oncogenic role of miR-155 in anaplastic large cell lymphoma lacking the t(2;5) translocation.The Journal of pathology · 2015Article
- STAT3 regulated ARF expression suppresses prostate cancer metastasis.Nature communications · 2015Article
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- Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue - a review.Diagnostic pathology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In targeted therapy, patient tumors are analyzed for aberrant activations of core cancer pathways, monitored based on biomarker expression, to ensure efficient treatment. Thus, diagnosis and therapeutic decisions are often based on the status of biomarkers determined by immunohistochemistry in combination with other clinical parameters. Standard evaluation of cancer specimen by immunohistochemistry is frequently impeded by its dependence on subjective interpretation, showing considerable intra- and inter-observer variability. To make treatment decisions more reliable, automated image analysis is an attractive possibility to reproducibly quantify biomarker expression in patient tissue samples. We tested whether image analysis could detect subtle differences in protein expression levels. Gene dosage effects generate well-graded expression patterns for most gene-products, which vary by a factor of two between wildtype and haploinsufficient cells lacking one allele. We used conditional mouse models with deletion of the transcription factors Stat5ab in the liver as well Junb deletion in a T-cell lymphoma model. We quantified the expression of total or activated STAT5AB or JUNB protein in normal (Stat5ab+/+ or JunB+/+), hemizygous (Stat5ab+/Δ or JunB+/Δ) or knockout (Stat5abΔ/Δ or JunBΔ/Δ) settings. Image analysis was able to accurately detect hemizygosity at the protein level. Moreover, nuclear signals were distinguished from cytoplasmic expression and translocation of the transcription factors from the cytoplasm to the nucleus was reliably detected and quantified using image analysis. We demonstrate that image analysis supported pathologists to score nuclear STAT5AB expression levels in immunohistologically stained human hepatocellular patient samples and decreased inter-observer variability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.