ArticleBioMed research international2014
Overexpression of D-xylose reductase (xyl1) gene and antisense inhibition of D-xylulokinase (xyiH) gene increase xylitol production in Trichoderma reesei.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Current trends in the production of xylitol and paving the way for metabolic engineering in microbes.Biotechnology for biofuels and bioproducts · 2025Review
- Engineering of Aspergillus niger for efficient production of D-xylitol from L-arabinose.Microbial cell factories · 2024Article
- Current genetic strategies to investigate gene functions in Trichoderma reesei.Microbial cell factories · 2023Review
- Biological production of xylitol by using nonconventional microbial strains.World journal of microbiology & biotechnology · 2022Review
- Production of xylitol by Saccharomyces cerevisiae using waste xylose mother liquor and corncob residues.Microbial biotechnology · 2021Article
- Transcription Analysis of RecombinantJournal of microbiology and biotechnology · 2020Article
- Identification of genes involved in xylose metabolism of Meyerozyma guilliermondii and their genetic engineering for increased xylitol production.AMB Express · 2020Article
- Deletion of either the regulatory geneBiotechnology for biofuels · 2019Article
- The NADPH Oxidases Nox1 and Nox2 Differentially Regulate Volatile Organic Compounds, Fungistatic Activity, Plant Growth Promotion and Nutrient Assimilation inFrontiers in microbiology · 2018Article
- The ACEII recombinant Trichoderma reesei QM9414 strains with enhanced xylanase production and its applications in production of xylitol from tree barks.Microbial cell factories · 2016Article
- Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei.Microbial cell factories · 2016Review
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5 authors.
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Abstract
T. reesei is an efficient cellulase producer and biomass degrader. To improve xylitol production in Trichoderma reesei strains by genetic engineering, two approaches were used in this study. First, the presumptive D-xylulokinase gene in T. reesei (xyiH), which has high homology to known fungi D-xylulokinase genes, was silenced by transformation of T. reesei QM9414 strain with an antisense construct to create strain S6-2-2. The expression of the xyiH gene in the transformed strain S6-2-2 decreased at the mRNA level, and D-xylulokinase activity decreased after 48 h of incubation. This led to an increase in xylitol production from undetectable levels in wild-type T. reesei QM9414 to 8.6 mM in S6-2-2. The T. reesei Δxdh is a xylose dehydrogenase knockout strain with increased xylitol production compared to the wild-type T. reesei QM9414 (22.8 mM versus undetectable). The copy number of the xylose reductase gene (xyl1) in T. reesei Δxdh strain was increased by genetic engineering to create a new strain Δ9-5-1. The Δ9-5-1 strain showed a higher xyl1 expression and a higher yield of xylose reductase, and xylitol production was increased from 22.8 mM to 24.8 mM. Two novel strains S6-2-2 and Δ9-5-1 are capable of producing higher yields of xylitol. T. reesei has great potential in the industrial production of xylitol.
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